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You are here:Home » Antibodies » Abs to Syntaxin, Synaptotagmin, Synaptophysin » Anti -Munc-18, phosphorylated (Ser515) (Mammalian Homolog of Unc-18, Munc18, hUNC18, Munc18-1, Munc18a, N-Sec1, p67, Rbsec1, Syntaxin Binding Protein 1, STXBP1, UNC18, UNC-18, Unc18 Homolog, Unc-18 Homolog, Unc 18-1, Unc-18-1, Unc18A, Unc-18A)

Anti -Munc-18, phosphorylated (Ser515) (Mammalian Homolog of Unc-18, Munc18, hUNC18,
Munc18-1, Munc18a, N-Sec1, p67, Rbsec1, Syntaxin Binding Protein 1, STXBP1, UNC18, UNC-18,
Unc18 Homolog, Unc-18 Homolog, Unc 18-1, Unc-18-1, Unc18A, Unc-18A)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B
Munc-18 (mammalian homologue of Unc-18) is a protein that is thought to be involved in regulating exocytosis due, at least in part to its ability to bind syntaxin (Ciufo et al., 2005). Munc18-1 is a neuron-specific member of the Sec1/Munc18 protein family that binds to syntaxin1A and is thought to stabilize the complex (Liu et al., 2004). The function of Munc-18 is thought to be regulated by PKC phosphorylation of Ser515 on the Munc-18 protein (Sassa et al., 1996).
Catalog #M2197-03
Positive Control Rat cortex lysate
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionOptimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
HostRabbit
SourceHuman
ConcentrationNot determined
Form10 mM Hepes (pH7.5), 150 mM NaCl, 100 ug/ml BSA and 50% glycerol
PurityPurified by immunoaffinity chromatography.
ImmunogenPhosphopeptide corresponding to amino acid residues surrounding the phospho- Ser515 of Munc-18. Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.
SpecificityThe antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will react with bovine, canine, chicken, human, mouse, non-human primates and Xenopus based on the fact that these species have 100% homology with the amino acid sequence used as antigen. Specific for the ~65k Munc-18 protein phosphorylated at Ser515. Immunolabeling is blocked by the phosphopeptide used as antigen but not by the corresponding dephosphopeptide.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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