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Mannitol Salt Agar (Powder)


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Selective medium for isolation and enumeration of Staphylococci. Recommended by USP for microbial limit tests. S. aureus strains produce colonies surrounded by yellow zones due to the fermentation of mannitol. Acid production is indicated by the phenol red dye. Non-pathogenic Staphylococci produce colonies surrounded by red or purple zones and are smaller in size. Most common organisms (Gram negatives) are inhibited by the high salt content.
Catalog #M2300-10
Pale pinkish beige to beige free-flowing powder.
SolubilityClear to slightly hazy, yellowish to light pink and complete
Cultural Response24-48 hrs (35°C)
Microbiological Analysis
Proteus mirabilisInhibited
Staphylococcus aureusGrowth, yellow colony
Staphylococcus epidermidisInhibited, red colony
Final pH (25°C)7.4 ± 0.2
Alternate namesSodium Chloride, Agar, D-Mannitol, Pancreatic Digest of Casein, Peptic Digest of Animal Tissue, Beef Extract, Phenol Red

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