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You are here:Home » Antibodies » Abs to Enzymes, Glycosidase » Anti -Mannosidase II

Anti -Mannosidase II

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Serum IH IC
Mannosidase II is a 135kD protein located on the luminal side of golgi membranes. The antibody is useful as a marker for golgi apparatus and for studing the fate of the golgi complex in diving cells.
Catalog #M2307
ApplicationsSuitable for use in Immunocytochemistry and Immunohistochemistry. Other applications have not been tested.
Recommended DilutionImmunocytochemistry: 1:100-1:400 on HeLa and COS7 cells fixed with ice-cold methanol. Formaldehyde fixation can reduce the staining intensity if left in contact with the cells for more than 15-20 minutes.
Immunohistochemistry: Cold methanol fixed tissue.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid from 0.02% sodium azide.
PuritySerum
ImmunogenPurified peptide from human mannosidase II.
SpecificityRecognizes human Mannosidase II. Useful marker for Golgi apparatus identification. Species Crossreactivity: Monkey. Does not crossreact with mouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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