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You are here:Home » Antibodies » Abs to Enzymes, Glycosidase » Anti -Mannosidase II (MAN2A1, alpha Mannosidase 2, alpha Mannosidase II, Golgi alpha Mannosidase II, MANA2, MANII, Mann II, Mannosidase alpha Class 2A Member 1, Mannosidase Two, Mannosyl Oligosaccharide 13 16 alpha Mannosidase)

Anti -Mannosidase II (MAN2A1, alpha Mannosidase 2, alpha Mannosidase II, Golgi alpha
Mannosidase II, MANA2, MANII, Mann II, Mannosidase alpha Class 2A Member 1, Mannosidase
Two, Mannosyl Oligosaccharide 13 16 alpha Mannosidase)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B
This gene encodes a protein which is a member of family 38 of the glycosyl hydrolases. The protein is located in the Golgi and catalyzes the final hydrolytic step in the asparagine-linked oligosaccharide (N-glycan) maturation pathway. Mutations in the mouse homolog of this gene have been shown to cause a systemic autoimmune disease similar to human systemic lupus erythematosus.
Catalog #M2307-05C
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionsELISA: 1:16,000
Western Blot: 1-3ug/ml
Optimal dilutions to be determined by the researcher.
Positive ControlHuman Placenta lysates
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 monthsat -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Peptide BlockingCorresponding peptide is available for Peptide Blocking studies. See M2307-05C-P. Antibody is typically 0.5mg/ml and peptide is supplied as a 100ul pellet. When peptide is reconstituted in 200ul water, the concentration would also be 0.5mg/ml. To start, the best ratio would be 1:1 (molar excess of peptide relative to antibody when identical volumes are mixed). Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. It is best is to have two identical blots to be incubated with equal amount of antibody, but one with the antibody pre-adsorbed to the peptide for 20min. Then incubate and develop the two blots in parallel.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in Tris, pH 7.3, 0.5% BSA, 0.02% sodium azide.
PurityPurified by affinity chromatography.
ImmunogenSynthetic peptide, C-KISSDIKSQNR, from the internal region of human MAN2A1.
SpecificityRecognizes human MAN2A1 at ~150kD.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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