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You are here:Home » Antibodies » Abs to Microtubule-associated Proteins » Anti -MAP2a,b,c (Microtubule Associated Protein 2a,b,c)

Anti -MAP2a,b,c (Microtubule Associated Protein 2a,b,c)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IH IF
Microtubule Associated Protein 2 exists in two high molecular weight forms (2a & 2b) and a low molecular weight form. The expression of MAP2 is developmentally regulated and its multiple forms arise by alternative splicing of a single gene. This antibody is useful in studies of neuron structure in normal and malignant brain tissue and in Alzheimerís tissue.
Catalog #M2350-01B
ApplicationsSuitable for use in Immunofluorescence, Western Blot and Immunohistochemistry. Other applications have not been tested.
Recommended DilutionsWestern Blot: 1-2ug/ml for 2 hours at RT.
Immunohistochemistry (Formalin/paraffin): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes.
Optimal dilutions to be determined by the researcher.
Positive ControlIMR-5 or T98G human glioblastoma cells or brain
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1
Clone No0.N.395
HostMouse
SourceBovine
Concentration~0.2mg/ml
FormSupplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See M2350-01BX.
PurityPurified by Protein G affinity chromatography.
ImmunogenBovine brain MAP2. Cellular Localization: Cytoplasmic.
EpitopeAa1-151.
SpecificityRecognizes the phosphorylated form of bovine MAP2a,b,c and shows no crossreactivity with other MAPs, tau and tubulin. Recognizes MAP2a and MAP2b (~280kD doublet) and MAP2c (70kD). Reacts with dendrites and cell bodies of neurons. Species Crossreactivity: human, rat, Xenopus and quail.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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