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You are here:Home » Antibodies » Abs to Microtubule-associated Proteins » Anti -MAP2 (Microtubule Associated Protein 2, MAP-2)

Anti -MAP2 (Microtubule Associated Protein 2, MAP-2)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IH
The microtubule-associated protein 2 (MAP-2) is an abundant neuronal cytoskeletal protein that binds to tubulin and stabilizes microtubules. (Herzog and Weber, 1978). MAP-2 is essential for the development and maitenance of neuronal morphology (Matus, 1991). Three primary isoforms of MAP-2, the high molecular weight MAP2a and MAP2b, and the low molecular weight MAP2c (resulting from alternate splicing of the MAP2 gene), have been identified in neurours. (Chung et al., 1996). The low molecular weight isoform, MAP2c, is expressed in developing brain and is down-regulated during brain maturation, whereas the high molecular weight MAP2b is expressed in both developing and adult brain. The MAP2a appears only after brain maturation (Tucker, 1990). All these forms bind to microtubules through a domain near the carboxyl terminus that contains either three or four similar repeats of a 31-amino-acid motif (Lewis et al., 1988). MAP-2 together with MAP-4 and tau proteins belong to the family of thermostable proteins associated with microtubules.
Catalog #M2350-02F
ApplicationsSuitable for use in Western Blot and Immunohistochemistry. Other applications have not been tested.
Recommended Dilutions
Western Blot: 0.1ug/ml detects MAP-2 on 10ug of rat brain microsomal tissue lysate.
Immunohistochemistry (paraffin): 1:200 detects MAP-2 in rat brain tissue.
Optimal dilutions to be determined by the researcher.
Positive ControlRat brain microsomal tissue lysate
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceRat
Concentration~1mg/ml
FormSupplied as a liquid in Tris-glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
PurityPurified by affinity chromatography.
ImmunogenThree linear peptides corresponding to the N-terminal and central regions of rat MAP-2 (KLH).
SpecificityRecognizes rat MAP-2. Species sequence homology: mouse (94%), human (72%).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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