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Anti -MAP Kinase (CDK), phosphorylated Substrate (Thr) (MAPK/CDK Substrate, Phospho-(Thr))
Anti -MAP Kinase (CDK), phosphorylated Substrate (Thr) (MAPK/CDK Substrate, Phospho-(Thr))
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
E B IH |
|
| The MAPK and CDK family of serine/threonine protein kinases plays important roles in cell signaling and cell cycle control. These kinases phosphorylate threonine or serine followed by a proline residue. To study and discover new MAPK and CDK substrates, we have a monoclonal antibody that binds to phospho-threonine followed by proline. As determined by ELISA using a wide variety of phospho-Thr-Pro peptides, Phospho-(Thr) MAPK/CDK Substrate Monoclonal Antibody recognizes the phospho-Thr-Pro motif in a highly context-independent fashion. It also interacts with a broad range of phospho-Thr-Pro-containing proteins as determined by Western analysis of 2D gels of nocodazole treated Jurkat cell extracts. | | | Catalog # | M2351-98 | | Applications | Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. | | Recommended Dilution | ELISA: 1:1000 | | Western Blot: 1:5000 | | Immunohistochemistry (Paraffin): 1:50 | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgM | | Clone No | 3i17 | | Host | Mouse | | Source | Mouse | | Concentration | As reported. | | Form | Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol. | | Purity | Purified by Protein A affinity chromatography. | | Immunogen | Synthetic phosphothreonine- proline-containing peptides (KLH coupled). | | Specificity | Detects phospho-threonine only when followed by proline. It reacts with proteins/peptides phosphorylated on the Thr-Pro motif in an otherwise highly context-independent fashion. Does not crossreact with phospho-threonine in the absence of an adjacent proline. Does not crossreact with phospho-tyrosine, but does react with some phospho-serine peptides containing the phospho-serine-proline motif. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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