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You are here:Home » Antibodies » Abs to Protein Kinase Substrates » Anti -MAP Kinase (CDK), phosphorylated Substrate (Thr) (MAPK/CDK Substrate, Phospho-(Thr))

Anti -MAP Kinase (CDK), phosphorylated Substrate (Thr) (MAPK/CDK Substrate, Phospho-(Thr))


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Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IH
The MAPK and CDK family of serine/threonine protein kinases plays important roles in cell signaling and cell cycle control. These kinases phosphorylate threonine or serine followed by a proline residue. To study and discover new MAPK and CDK substrates, we have a monoclonal antibody that binds to phospho-threonine followed by proline. As determined by ELISA using a wide variety of phospho-Thr-Pro peptides, Phospho-(Thr) MAPK/CDK Substrate Monoclonal Antibody recognizes the phospho-Thr-Pro motif in a highly context-independent fashion. It also interacts with a broad range of phospho-Thr-Pro-containing proteins as determined by Western analysis of 2D gels of nocodazole treated Jurkat cell extracts.
Catalog #M2351-98
ApplicationsSuitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionELISA: 1:1000
Western Blot: 1:5000
Immunohistochemistry (Paraffin): 1:50
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
Clone No3i17
ConcentrationNot determined.
FormSupplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.
PurityPurified by Protein A affinity chromatography.
ImmunogenSynthetic phosphothreonine- proline-containing peptides (KLH coupled).
SpecificityDetects phospho-threonine only when followed by proline. It reacts with proteins/peptides phosphorylated on the Thr-Pro motif in an otherwise highly context-independent fashion. Does not crossreact with phospho-threonine in the absence of an adjacent proline. Does not crossreact with phospho-tyrosine, but does react with some phospho-serine peptides containing the phospho-serine-proline motif.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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