| MAPKAP kinase-2 (or MK2) is an acronym for mitogen-activated protein kinase-activated protein |
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| kinase-2. This enzyme is phosphorylated and activated by Erks and p38 MAPK in vitro. While multiple residues of MK2 are phosphorylated in vivo in response to stress, only four of the |
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| residues Thr25, Thr222, Ser272 and Thr334 are phosphorylated by p38 in an in vitro kinase assay. MK2 is serine-threonine protein kinase that contains a proline rich sequence and two |
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| putative SH3-binding sites. MK2 phosphorylates downstream components on a consensus Pro-X-Ser/Thr-Pro motif. The bestcharacterized MK2 substrate is hsp27. Although unknown, the |
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| physiological function of MK2 may be to increase stress resistance. MK2 is an essential component in the inflammatory response that regulates biosynthesis of TNF-a at a posttranscriptional level and MK2 is rapidly phosphorylated and activated in response to cytokines, stress and chemotactic factors. |
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| Catalog # | M2355-01A |
| See Control Peptide M2355-01A1 |
| Applications | Suitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications not tested. |
| Recommended Dilution | ELISA: 1:15000 |
| Western Blot: 1:2000 |
| Optimal dilutions to be determined by the researcher. |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add glycerol (40-50%)aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Source | Human |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide. |
| Purity | Purified by immunoaffinity chromatography. |
| Immunogen | Synthetic peptide corresponding to aa 310-325 of MAPKAP Kinase 2 (MTSALATMRVDYEQIK) conjugated to KLH using maleimide. A terminal cysteine residue was added to facilitate coupling. |
| Specificity | Recognizes MAPKAP. This sequence is conserved 100% in human, mouse, rabbit, rat, hamster, chicken, Xenopus and zebrafish. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
| Alternate names | EC=2.7.11.1 |
