| MAP kinase kinase 6 (Mek6/MKK6) is a 38 kD dual-specificity protein kinase that is abundant in skeletal muscle and is activated by UV light, anisomycin, osmotic shock and inflammatory cytokines, but not by phorbol esters, NGF or EGF (1). Mek6 phosphorylates and activates MAP kinases such as p38 (2,3) and p38 beta (4). Mek6 is phosphorylated and activated by ASK1, a MAPKKK that induces apoptosis when overexpressed (5). Mek6 also interacts with mixed lineage kinase-3 (MLK-3), a kinase that participates in stress response kinase cascade, possibly by phosphorylating MKK4, an isoform of Mek6 (6). |
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| Applications: |
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| Western Blot (Colorimetric): 4ug/ml |
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| Immunoprecipitation: 4ug/ml |
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| Optimal dilutions to be determined by researcher. |
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| Positive Controls: |
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| HeLa Cell Lysate (Heat Shocked), |
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| Rat Brain Tissue Extract, |
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| Mouse Brain Tissue Extract |
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| Catalog # | M2363-22B |
| Clone Type | Polyclonal |
| Host | Rabbit |
| Source | Human |
| Form | Supplied as a liquid in borate buffered saline, pH 8.2 25mM sodium borate, 100mM boric acid, 75mM sodium chloride, 5mM EDTA (disodium salt). |
| Purity | Purified by immunoaffinity chromatography. |
| Immunogen | A 15 residue synthetic peptide (C)KGTDVASFVKLILGD based on |
| the human Mek6 (residues 320-334) with the cysteine (C) residue added and the peptide coupled to KLH |
| Specificity | The rabbit immunoaffinity purified antibody recognizes an ~38kD protein corresponding to the apparent molecular mass of Mek6 on SDS-PAGE immunoblots, in samples of human, rat, mouse and Xenopus origin. Antibody specificity is confirmed by peptide inhibition studies. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
