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You are here:Home » Antibodies » Abs to Transcription Factors, Zinc (Ring) Finger » Anti -MARCH8 (E3 Ubiquitin-protein Ligase MARCH8, Membrane-associated RING Finger Protein 8, Membrane-associated RING-CH Protein VIII, MARCH-VIII, RING Finger Protein 178, Cellular Modulator Of Immune Recognition, c-MIR, MARCH8, MIR, RNF178)

Anti -MARCH8 (E3 Ubiquitin-protein Ligase MARCH8, Membrane-associated RING Finger Protein
8, Membrane-associated RING-CH Protein VIII, MARCH-VIII, RING Finger Protein 178, Cellular
Modulator Of Immune Recognition, c-MIR, MARCH8, MIR, RNF178)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IC
MARCH8 also called MIR (modulator of immune recognition), or RNF178 (ring finger 178), is a 33kD two-pass transmembrane protein of lysosomal and early endosomal membranes in the MARCH family. MARCH proteins are E3 ubiquitin ligases that promote endocytosis and degradation of their target proteins. MARCH8 ubiquitinates B7-2/CD86 and probably Fas, Tfr and MHC class II proteins such as HLA-A2.1. Within the cytoplasmic C- terminal region used as an immunogen, human MARCH8 shares 87% aa identity with mouse and rat MARCH8.
Catalog #M2374-88C
ApplicationsSuitable for use in Western Blot and Immunocytochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 0.1ug/ml
Immunocytochemistry: 8-25ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2B
Clone No12K136
HostMouse
SourceHuman
Concentration~0.5mg/ml after reconstitution
FormSupplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 0.5mg/ml.
PurityPurified by Protein A/G affinity chromatography.
ImmunogenRecombinant corresponding to aa218-291 from human MARCH8 expressed in E.coli.
SpecificityRecognizes human MARCH8. Species Crossreactivity: Mouse and rat. Does not react with recombinant human MARCH1 or rhGRAIL.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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