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You are here:Home » Antibodies » Abs to Protein Kinase Substrates » Anti -MARCKS (Myristolated Alanine Rich C Kinase Substrate, Myristoylated Alanine Rich Protein Kinase C Substrate, MACS, MARCS, 80K-L Protein, 81kD Protein Light Chain, MGC52672, Phosphomyristin, PKCSL, PRKCSL, Protein Kinase C Substrate)

Anti -MARCKS (Myristolated Alanine Rich C Kinase Substrate, Myristoylated Alanine Rich
Protein Kinase C Substrate, MACS, MARCS, 80K-L Protein, 81kD Protein Light Chain,
MGC52672, Phosphomyristin, PKCSL, PRKCSL, Protein Kinase C Substrate)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Ascites E B IP
Catalog #M2375
ApplicationsSuitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications have not been tested.
Recommended DilutionWestern Blot: 1:500-1:1000. Detects MARCKS in a bovine brain extract. Bovine brain extract was resolved by electrophoresis, transferred to nitrocellulose and probed with M2375 (1:500). Proteins were visualized using an IgG (HRP) goat anti-mouse* and a chemiluminescence detection system.
Immunoprecipitation: Precipitates MARCKS proteins from bovine brain extracts. Used mainly for the evaluation of MARCKS protein phosphorylation in intact human cells. Determined by immunoprecipitation of 32P-labeled protein as an indicator of protein kinase C activation in cells.
Optimal dilutions to be determined by the researcher.
*Recommended secondary antibodiesI1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo
I1904-06H IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1
Clone No0.T.89
HostMouse
SourceHuman
ConcentrationNot determined.
FormSupplied as a liquid in 0.05% sodium azide.
PurityAscites.
ImmunogenRecombinant protein corresponding to human MARCKS at C-terminal.
SpecificityRecognizes human MARCKS. Species Crossreactivity: Bovine. Does not crossreact with rat, mouse or chicken.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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