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You are here:Home » Antibodies » Abs to Protein Kinase Substrates » Anti -MARCKS, phosphorylated (Ser159, Ser163) (Myristolated Alanine Rich C Kinase Substrate, Myristoylated Alanine Rich Protein Kinase C Substrate, MACS, MARCS, 80K-L Protein, 81kD Protein Light Chain, MGC52672, Phosphomyristin, PKCSL, PRKCSL, Protein Kinase C

Anti -MARCKS, phosphorylated (Ser159, Ser163) (Myristolated Alanine Rich C Kinase
Substrate, Myristoylated Alanine Rich Protein Kinase C Substrate, MACS, MARCS, 80K-L
Protein, 81kD Protein Light Chain, MGC52672, Phosphomyristin, PKCSL, PRKCSL, Protein
Kinase C

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Rabbit Supernatant B IP
The expression of a major cellular substrate for protein kinase C, the MARCKS protein, is regulated in a cell-, tissue-, and developmental stage-specific fashion; in addition, this expression can be stimulated acutely by various cytokines in certain cell types (1). As a filamentous (F) actin crosslinking protein, MARCKS binds to actin, calmodulin, and synapsin. In murine, MARCKS binding activity is regulated by PKC phosphorylation at Ser152, 156, and 163 of MARCKS during macrophage and neutrophil activation, growth factor-dependent mitogenesis and neurosecretion. After phospholyation, MARCKS is redistributed from plasma membrane to cytoplasm and is involved in leukocyte motility. MARCKS may be a regulated crossbridge between actin and the plasma membrane, and modulation of the actin crosslinking activity of the MARCKS protein by calmodulin and phosphorylation represents a potential convergence of the calcium-calmodulin and PKC signal transduction pathways in the regulation of the actin cytoskeleton (2).
Catalog #M2375-05F
ApplicationsSuitable for use in Western Blot and Immunoprecipitation. Other applications not tested.
Recommended DilutionWestern Blot: 1:10001:2000
Immunoprecipitation: 1:40
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Manufactured incorporating RabMAb® technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
Clone TypeMonoclonal
IsotypeIgG
Clone No9H458
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in 50mM Tris-Glycine, pH 7.4, 0.15M sodium chloride, 40% Glycerol, 0.01% sodium azide, 0.05% BSA.
PuritySupernatant
ImmunogenSynthetic phospho-peptide corresponding to residues surrounding serine 159 and 163 of human MARCKS protein.
SpecificityRecognizes human MARCKS phosphorylated at serine 159 and serine 163. Also recognizes the orthologous phosphorylation sites in mouse and rat is serine 152 and 156.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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