Anti -MARCKS (Myristolated Alanine Rich C Kinase Substrate, Myristoylated Alanine Rich
Protein Kinase C Substrate, MACS, MARCS, 80K-L Protein, Protein Kinase C Substrate, 80 kD
Protein Light Chain, PKCSL, PRKCSL)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Affinity Purified |
B |
|
| Myristoylated Alanine-Rich C Kinase Substrate (MARCKS) a major in vivo substrate of protein kinase C as well as of proline-directed protein kinases such as mitogen-activated protein kinase , contains a highly conserved N-terminal domain with an N-terminal myristoyl group and a basic phosphorylation domain in the middle of the molecule that is at the same time the binding sites for calmodulin. The phosphorylation of the latter domain results in the disruption of the calmodulin binding of the domain. The protein binds to phospholipid membrane through both the N-terminal myristoyl moiety and the phosphorylation domain of basic amphiphilic nature. Protein kinase C-dependent phosphorylation introduces negative charges into the positively charged effector domain, consequently neutralizing its interaction with acidic phospholipids and promoting its release from the membrane. The protein plays a major role in cell motility, phagocytosis, membrane trafficking and mitogenesis. human MARCKS gene is localized on human chromosome 6q22.2. | | | Catalog # | M2375-05L | | Applications | Suitable for use in Western Blot. Other applications not tested. | | Recommended Dilution | Western Blot: 1:500-1:1000 | | Optimal dilutions to be determined by the researcher. | | Positive Control | NIH/3T3 cells | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Rabbit | | Source | Human | | Concentration | As reported | | Form | Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol. | | Purity | Purified by immunoaffinity chromatography via sequential chromatography on phospho and dephosphopeptide affinity columns | | Immunogen | Synthetic non-phosphopeptide corresponding to human MARCKS around the phosphorylation site of serine 158 (R-F-S-F-K). | | Specificity | Detects total levels of endogenous human MARCKS, including both non-phosphorylated and phosphorylated forms. Species crossreactivity: rat and mouse. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. | | Alternate names | FLJ14368, FLJ90045 |
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