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You are here:Home » Antibodies » Abs to Protein Kinase Substrates » Anti -Marcks, Phosphorylated (Ser152/Ser156) (Myristoylated Alanine-rich C-kinase Substrate, MARCKS, Protein Kinase C Substrate 80kD Protein, Macs)

Anti -Marcks, Phosphorylated (Ser152/Ser156) (Myristoylated Alanine-rich C-kinase
Substrate, MARCKS, Protein Kinase C Substrate 80kD Protein, Macs)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B
MARCKS (Myristoylated alanine-rich protein kinase C substrate) is a major PKC substrate that is distributed in various cell types. MARCKS has been implicated in cell motility, cell adhesion, phagocytosis, membrane traffic and mitogenesis. PKC phosphorylates Ser152, 156 and 163 of MARCKS, which regulates MARCKS's calcium/calmodulin binding activity and filamentous (F)-actin cross-linking activity. In addition, phosphorylation by PKC results in translocation of MARCKS from membrane to cytoplasm.
Catalog #M2375-05M
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1:200-1:1000
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceRat
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 1% BSA, 0.02% thimerosal, 50% glycerol.
PurityPurified by affinity chromatography.
ImmunogenSynthetic peptide corresponding to aa surrounding Ser152/156 from rat MARCKS.
SpecificityRecognizes rat MARCKS when phosphorylated at Ser152/156. Species Crossreactivity: mouse and human.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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