Anti -MARCKS (Myristolated Alanine Rich C Kinase Substrate, Myristoylated Alanine Rich
Protein Kinase C Substrate, MACS, MARCS, 80K-L Protein, 81kD Protein Light Chain,
MGC52672, Phosphomyristin, PKCSL, PRKCSL, Protein Kinase C Substrate)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Rabbit |
Supernatant |
B IH IC FC |
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| The expression of a major cellular substrate for protein kinase C, the MARCKS protein, is regulated in a cell-, tissue-, and developmental stage-specific fashion; in addition, this expression can be stimulated acutely by various cytokines in certain cell types (1). MARCKS) binds calmodulin and is a predominant, specific substrate of PKC which is phosphorylated during macrophage and neutrophil activation, growth factor-dependent mitogenesis and neurosecretion; it is redistributed from plasma membrane to cytoplasm when phosphorylated and is involved in leukocyte motility. It has been reported that MARCKS is a filamentous (F) actin crosslinking protein, with activity that is inhibited by PKC-mediated phosphorylation and by binding to calcium-calmodulin. MARCKS may be a regulated crossbridge between actin and the plasma membrane, and modulation of the actin crosslinking activity of the MARCKS protein by calmodulin and phosphorylation represents a potential convergence of the calcium-calmodulin and PKC signal transduction pathways in the regulation of the actin cytoskeleton (2). | | | Catalog # | M2375-08D | | Applications | Suitable for use in Flow Cytometry, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested. | | Recommended Dilution | Flow Cytometry: 1:10 | | Western Blot: 1:10,000 | | Immunohistochemistry: 1:100-1:250 | | Immunocytochemistry: 1:100-1:250 | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG | | Clone No | 9H459 | | Host | Rabbit | | Source | Human | | Concentration | As reported | | Form | Supplied as a liquid in 50mM Tris-Glycine, pH 7.4, 0.15M sodium chloride, 40% Glycerol, 0.01% sodium azide, 0.05% BSA. | | Purity | Supernatant | | Immunogen | Synthetic peptide corresponding to residues on human MARCKS. | | Specificity | Recognizes human MARCKS. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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Download Specifications for Anti -MARCKS (Myristolated Alanine Rich C Kinase Substrate, Myristoylated Alanine Rich Protein Kinase C Substrate, MACS, MARCS, 80K-L Protein, 81kD Protein Light Chain, MGC52672, Phosphomyristin, PKCSL, PRKCSL, Protein Kinase C Substrate) |
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