MARKL1, a MARK type protein kinase, contains a N-terminal serine/threonine kinase domain, a central ubiquitin-associated domain and a C-terminal KA1-associated kinase domain. A smaller mRNA transcript, lacking exon 16 containing the KA1-associated kinase domain, also has been reported.
Applications
Suitable for use in ELISA, Immunofluorescence and Immunohistochemistry. Other applications not tested.
Recommended Dilution
ELISA: 1:40,000 Immunofluorescence: 1:100-1:500 Immunohistochemistry (Formalin fixed paraffin embedded): 10ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to human MARK4.
Form
Supplied as a liquid in PBS (without Mg2+, Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human MARK4. Species sequence homology: mouse.
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