| MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Seven other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1 and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum. |
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| Catalog # | M2410 |
| Applications | Suitable for use in Flow Cytometry, Immunoprecipitation, Western Blot and Immunohistochemistry. Other applications have not been tested. |
| Recommended Dilutions | Immunoprecipitation (Denatured verified): 2ug/mg protein lysate. Use Protein A. |
| Western Blot: 0.5-1ug/ml for 2 hours at RT. |
| Immunohistochemistry (Formalin/paraffin): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. M2410-12 is preferred for this application. |
| Optimal dilutions to be determined by the researcher. |
| Positive Control | CaC1 melanoma cells and melanomas |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG2b,k |
| Clone No | 0.N.396 |
| Host | Mouse |
| Source | Human |
| Concentration | ~0.2mg/ml |
| Form | Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See M2410X. |
| Purity | Purified by Protein A affinity chromatography. |
| Immunogen | Recombinant human MART-1 protein. |
| Cellular Localization | Cytoplasmic |
| Specificity | Recognizes human MART-1 at ~18kD. Labels melanomas and other tumors showing melanocytic differentiation. Does not label tumor cells of epithelial, lymphoid, glial or mesenchymal origin. Species Crossreactivity: Equine. Does not crossreact with mouse or rat. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
