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You are here:Home » Antibodies » Abs to Cancer Markers » Anti -MART-1 (Melanoma Antigen Recognized by T-cells 1, Melan-A)

Anti -MART-1 (Melanoma Antigen Recognized by T-cells 1, Melan-A)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IP IH FC
MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Seven other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1 and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum.
Catalog #M2410
ApplicationsSuitable for use in Flow Cytometry, Immunoprecipitation, Western Blot and Immunohistochemistry. Other applications have not been tested.
Recommended DilutionsImmunoprecipitation (Denatured verified): 2ug/mg protein lysate. Use Protein A.
Western Blot: 0.5-1ug/ml for 2 hours at RT.
Immunohistochemistry (Formalin/paraffin): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. M2410-12 is preferred for this application.
Optimal dilutions to be determined by the researcher.
Positive ControlCaC1 melanoma cells and melanomas
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG2b,k
Clone No0.N.396
HostMouse
SourceHuman
Concentration~0.2mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See M2410X.
PurityPurified by Protein A affinity chromatography.
ImmunogenRecombinant human MART-1 protein.
Cellular LocalizationCytoplasmic
SpecificityRecognizes human MART-1 at ~18kD. Labels melanomas and other tumors showing melanocytic differentiation. Does not label tumor cells of epithelial, lymphoid, glial or mesenchymal origin. Species Crossreactivity: Equine. Does not crossreact with mouse or rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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