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You are here:Home » Antibodies » Antibodies-Protease Inhibitors » Anti -Maspin (SERPINB5 , Serpin Peptidase inhibitor, Clade B (ovalbumin), member 5, Peptidase inhibitor 5, PI5, PI-5, Serpin B5)

Anti -Maspin (SERPINB5 , Serpin Peptidase inhibitor, Clade B (ovalbumin), member 5,
Peptidase inhibitor 5, PI5, PI-5, Serpin B5)


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Clone Host Grade Applications
Monoclonal Rat Affinity Purified E B IP
Serpin B5, also known as Maspin, is a secreted member of the Serpin family although it has no known serine protease inhibitor activity. It is produced by mammary epithelial cells and functions as a tumor suppressor. Serpin B5 is a valuable biomarker of tumor progression and overall prognosis.
Catalog #M2412-50D
ApplicationsSuitable for use in Direct ELISA, Western Blot, and Immunoprecipitation. Other applications not tested.
Recommended DilutionsWestern Blot: 1-2ug/ml. The detection limit for recombinant human Maspin is ~100ng/lane under non-reducing conditions and 25ng/lane under reducing conditions.
Immunoprecipitation: 25ug/ml. Conditioned cell culture medium spiked with recombinant human Maspin
Direct ELISA: 0.5-1ug/ml. The detection limit for recombinant human Maspin is ~15ng/well
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
Clone No9H158
Concentration~0.5mg/ml (after reconstitution)
FormSupplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
PurityPurified by Protein G affinity chromatography.
ImmunogenRecombinant protein corresponding to aa1-375 of human Maspin expressed in E. coli (Accession #NP_002630).
SpecificityRecognizes human Maspin. Does not react with recombinant human Maspin A5, B6, B8 or B9.
Species Sequence HomologyChimpanzee, 99%; canine, 92%; mouse, 89%; rat, 88%; chicken, 64%
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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