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You are here:Home » Antibodies » Abs to Protease Inhibitors » Anti -Maspin (SERPINB5 , Serpin Peptidase inhibitor, Clade B (ovalbumin), member 5, Peptidase inhibitor 5, PI5, PI-5, Serpin B5)

Anti -Maspin (SERPINB5 , Serpin Peptidase inhibitor, Clade B (ovalbumin), member 5,
Peptidase inhibitor 5, PI5, PI-5, Serpin B5)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Rat Affinity Purified E B IP
Serpin B5, also known as Maspin, is a secreted member of the Serpin family although it has no known serine protease inhibitor activity. It is produced by mammary epithelial cells and functions as a tumor suppressor. Serpin B5 is a valuable biomarker of tumor progression and overall prognosis.
Catalog #M2412-50D
ApplicationsSuitable for use in Direct ELISA, Western Blot, and Immunoprecipitation. Other applications not tested.
Recommended DilutionsWestern Blot: 1-2ug/ml. The detection limit for recombinant human Maspin is ~100ng/lane under non-reducing conditions and 25ng/lane under reducing conditions.
Immunoprecipitation: 25ug/ml. Conditioned cell culture medium spiked with recombinant human Maspin
Direct ELISA: 0.5-1ug/ml. The detection limit for recombinant human Maspin is ~15ng/well
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2b
Clone No9H158
HostRat
SourceHuman
Concentration~0.5mg/ml (after reconstitution)
FormSupplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
PurityPurified by Protein G affinity chromatography.
ImmunogenRecombinant protein corresponding to aa1-375 of human Maspin expressed in E. coli (Accession #NP_002630).
SpecificityRecognizes human Maspin. Does not react with recombinant human Maspin A5, B6, B8 or B9.
Species Sequence HomologyChimpanzee, 99%; canine, 92%; mouse, 89%; rat, 88%; chicken, 64%
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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