Anti -Maspin (SERPINB5 , Serpin Peptidase inhibitor, Clade B (ovalbumin), member 5,
Peptidase inhibitor 5, PI5, PI-5, Serpin B5)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Rat |
Affinity Purified |
E B IP |
|
| Serpin B5, also known as Maspin, is a secreted member of the Serpin family although it has no known serine protease inhibitor activity. It is produced by mammary epithelial cells and functions as a tumor suppressor. Serpin B5 is a valuable biomarker of tumor progression and overall prognosis. | | | Catalog # | M2412-50D | | Applications | Suitable for use in Direct ELISA, Western Blot, and Immunoprecipitation. Other applications not tested. | | Recommended Dilutions | Western Blot: 1-2ug/ml. The detection limit for recombinant human Maspin is ~100ng/lane under non-reducing conditions and 25ng/lane under reducing conditions. | | Immunoprecipitation: 25ug/ml. Conditioned cell culture medium spiked with recombinant human Maspin | | Direct ELISA: 0.5-1ug/ml. The detection limit for recombinant human Maspin is ~15ng/well | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG2b | | Clone No | 9H158 | | Host | Rat | | Source | Human | | Concentration | ~0.5mg/ml (after reconstitution) | | Form | Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 200ul sterile PBS. | | Purity | Purified by Protein G affinity chromatography. | | Immunogen | Recombinant protein corresponding to aa1-375 of human Maspin expressed in E. coli (Accession #NP_002630). | | Specificity | Recognizes human Maspin. Does not react with recombinant human Maspin A5, B6, B8 or B9. | | Species Sequence Homology | Chimpanzee, 99%; canine, 92%; mouse, 89%; rat, 88%; chicken, 64% | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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