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You are here:Home » Antibodies » Antibodies-Protease Inhibitors » Anti -Maspin (Serpin peptidase inhibitor, clade B (ovalbumin), member 5, Peptidase inhibitor 5, PI5, PI-5, Serpin B5)

Anti -Maspin (Serpin peptidase inhibitor, clade B (ovalbumin), member 5, Peptidase
inhibitor 5, PI5, PI-5, Serpin B5)


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Clone Host Grade Applications
Monoclonal Mouse Purified E B IF
Serine (or cysteine) proteinase inhibitor clade B (ovalbumin) member 5, also known as Maspin, is a tumor suppressor protein of serine proteinase inhibitor family. It plays an essential role in embryonic development through critical roles in cell adhesion. Also, it is present in normal breast and prostatic epithelial cells but downregulated in the respective carcinomas.
Catalog #M2412-50F
ApplicationsSuitable for use in Immunofluorescence, ELISA and Western Blot. Other applications not tested.
Recommended DilutionsImmunofluorescence: 1:500 detects Maspin in human HeLa cells
Western Blot: 1:1000-1:2000. Cell lysates of HeLa cells (20ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with M21412-50F (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP* and an ECL detection system.
Optimal dilutions to be determined by researcher.
*Recommended Secondary AntibodiesI1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo
I1904-06H: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo
HybridomaMouse F0 myeloma cells with spleen cells from BALB/c mice.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
Clone No10K402
FormSupplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenRecombinant corresponding to aa1-375 from human Maspin purified from E. coli (NP_002630).
SpecificityRecognizes human Maspin.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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