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You are here:Home » Antibodies » Abs to Cell Markers » Anti -Mast Cell Chymase

Anti -Mast Cell Chymase

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IH
Binds to human mast cell chymase, an important marker of mast cells and also an important mediator of inflammation. May be used to detect mast cells in routinely fixed tissues and has been used to study the distribution of mast cells in skin, synovium, lung and heart. Binding to chymase is not reduced in the presence of heparin, suggesting that the antibody does not bind to the heparin-binding domain of chymase.
Catalog #M2414-01A
ApplicationsSuitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionsImmunohistochemistry (Paraffin): 1:500-1:1000. Does not require protein digestion pretreatment or antigen retrieval. Also suitable for use in frozen sections.
Optimal dilutions to be determined by the researcher.
Recommended Secondary AntibodiesI1904-65W: IgG, F(abí)2 (HRP) Pab RbxMo
I1904-66C: IgG, F(abí)2, X-Adsorbed (HRP) Pab RbxMo
Recommended Negative ControlI1904-78R: IgG1 Murine Negative Control
Recommended Positive Control TissueSkin
Hybridoma
P3/NS1/AG4.1 myeloma cells with spleen cells from Balb/c mice.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1
Clone No3H2641
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.09% sodium azide
PurityPurified by Protein G affinity chromatography
ImmunogenPurified human skin chymase
SpecificityRecognizes human mast cell chymase
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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