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You are here:Home » Antibodies » Abs to Cell Markers » Anti -Mast Cell Chymase (CMA1)

Anti -Mast Cell Chymase (CMA1)

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Specifications

Clone Host Grade Applications
Polyclonal Sheep Affinity Purified E B IP
Chymase (also mast cell protease I) is a secreted, 30kD glycoprotein member of the granzyme subfamily, peptidase S1 family of enzymes. It is secreted by mast cells and vascular smooth muscle cells, and converts angiotensin I into angiotensin II. This promotes fibroblast proliferation and secretion of ECM. Mature human chymase is 226aa in length. It contains one peptidase S1 domain (aa22-245). There is one potential alternate splice form. It shows a deletion of the 99 C-terminal amino acids (aa49-247).
Catalog #M2414-01B
ApplicationsSuitable for use in Western Blot, Immunoprecipitation and ELISA. Other applications not tested.
Recommended DilutionsWestern Blot: 0.1ug/ml using recombinant human Chymase 1
Immunoprecipitation: 25ug/ml using rhChymase
from conditioned media of transfected NS0 cells.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C after receipt. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. 
Clone TypePolyclonal
IsotypeIgG
HostSheep
SourceHuman
Concentration~0.2mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from PBS, ph 7.4, 5% trehalose. Reconstitute with 500ul sterile PBS.
PurityPurified by immunoaffinity chromatography.
ImmunogenRecombinant protein corresponding to aa20-247 from human Chymase expressed in NS0 cells (P23946).
SpecificityRecognizes human Chymase. Crossreactivity: ~10% with MCPT1 and <1% with rmMCPT6 and rmMCPT7.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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