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You are here:Home » Antibodies » Abs to Cell Markers » Anti -Mast Cell Chymase (CMA1, Chymase 1, Chymase 1 Mast Cell, Chymase Heart, CYH, CYM, EC 3.4.21.39, Mast Cell Protease I, MCT1, MGC119890, MGC119891)

Anti -Mast Cell Chymase (CMA1, Chymase 1, Chymase 1 Mast Cell, Chymase Heart, CYH, CYM, EC
3.4.21.39, Mast Cell Protease I, MCT1, MGC119890, MGC119891)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Purified B IH
Mast cells contain a number of preformed chemical mediators such as histamine, chymase, carboxypeptidase and proteolytic tryptase. Human Mast Cell Chymase is considered to be an important marker of mast cells as well as an important mediator of inflammation.
Catalog #M2414-05A
ApplicationsSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionImmunohistochemistry: 1:100-1:200 for 30 min. at RT using ABC method. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10mM citrate buffer, pH 6.0 prior to immunostaining.
Optimal dilutions to be determined by the researcher.
Positive ControlTonsil
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No8L679
HostMouse
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in PBS with sodium azide
PurityPurified
ImmunogenPurified human skin Chymase
SpecificityRecognizes human Chymase. Reacts with mast cells distributed in skin, synovium, lung and heart.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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