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You are here:Home » Antibodies » Abs to Enzymes, Protease (Proteinase, Peptidase) » Anti -Mast Cell Tryptase

Anti -Mast Cell Tryptase

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IH
Mast cells contain a number of preformed chemical mediators such as histamine, chymase, carboxypeptidase and proteolytic tryptase. A substantial quantity of tryptase is estimated to be found in mast cells of skin and lung and suggestes this enzyme plays a major role in mast cell mediated events. In vitro studies indicate tryptase can cleave C3 to form C3a anaphylatoxin, inactivate fibrinogen as a coaguable substrate for thrombin and activate latent collagenase. Human mast cell tryptase has considerable potential as a marker for mast cell activation as well as being a mediator of inflammation.
Catalog #M2415-01
ApplicationsSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1-2ug/ml for 2hrs at RT.
Immunohistochemistry (Formalin/paraffin) 0.06-0.12ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
Optimal dilutions to be determined by the researcher.
HybridomaProduced by fusion between BALB/c splenocytes and myeloma NS1 cells.
Cellular LocalizationCytoplasmic
Positive ControlTonsil
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No1.N.5
HostMouse
SourceHuman
Concentration~0.1mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide, before the addition of glycerol to 40%. Also available without BSA and azide. Refer to cat # M2415-01X.
PurityPurified by Protein G affinity chromatoraphy.
ImmunogenHuman mast cell tryptase isolated from purified human lung tissues
SpecificityReacts with Mast Cell Tryptase, both a and b isoforms. Species Crossreactivity: Human, monkey, canine and feline.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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