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You are here:Home » Antibodies » Abs to Enzymes, Protease (Proteinase, Peptidase) » Anti -Mast Cell Tryptase (BSA & Azide Free)

Anti -Mast Cell Tryptase (BSA & Azide Free)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IH
Mast cells contain a number of preformed chemical mediators such as histamine, chymase, carboxypeptidase and proteolytic tryptase. A substantial quantity of tryptase is estimated to be found in mast cells of skin and lung and suggestes this enzyme plays a major role in mast cell mediated events. In vitro studies indicate tryptase can cleave C3 to form C3a anaphylatoxin, inactivate fibrinogen as a coaguable substrate for thrombin and activate latent collagenase. Human mast cell tryptase has considerable potential as a marker for mast cell activation as well as being a mediator of inflammation.
Catalog #M2415-01X
ApplicationsSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionsWestern Blot: 1:50 for 2 hours at RT.
Immunohistochemistry (Formalin/paraffin):1:2000 for 20 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6, for 10-20 minutes followed by cooling at RT for 20 minutes.
Optimal dilutions to be determined by the researcher.
Recommended Positive ControlTonsil
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1
Clone No1.N.5
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.4. Also available with BSA and sodium azide. See M2415-01.
PurityPurified by Protein G affinity chromatography.
ImmunogenHuman mast cell tryptase purified from human lung tissues. Cellular Localization: Cytoplasmic.
SpecificityRecognizes human Mast Cell Tryptase. Does not bind to any other cell type in Immunohistochemistry. Species Crossreactivity: monkey, canine and feline.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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