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You are here:Home » Antibodies » Abs to Enzymes, Protease (Proteinase, Peptidase) » Anti -Mast Cell Tryptase

Anti -Mast Cell Tryptase

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IH IF
Mast cells contain a number of preformed chemical mediators such as histamine, chymase, carboxypeptidase and proteolytic tryptase. A substantial quantity of tryptase is estimated to be found in mast cells of skin and lung and suggestes this enzyme plays a major role in mast cell mediated events. In vitro studies indicate tryptase can cleave C3 to form C3a anaphylatoxin, inactivate fibrinogen as a coaguable substrate for thrombin and activate latent collagenase. Human mast cell tryptase has considerable potential as a marker for mast cell activation as well as being a mediator of inflammation.
Catalog #M2415-02C
ApplicationsSuitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry. Other applications not tested.
Recommended DilutionsImmunohistochemistry: Frozen sections
Immunohistochemistry (Paraffin): 1:10,000. Requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer, pH 6.0 is recommended.
Optimal dilutions to be determined by the researcher.
Recommended Secondary AntibodiesI1904-65W: IgG, F(abí)2 (HRP) Pab RbxMo
I1904-66C: IgG, F(abí)2, X-Adsorbed (HRP) Pab RbxMo
HybridomaNS1 myeloma cells with spleen cells from Balb/c mice.
Immunohistochemistry Positive Control TissueTonsil
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Clone TypeMonoclonal
IsotypeIgG1
Clone No3H2643
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, 0.09% sodium azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenHuman mast cell tryptase purified from human lung tissue.
SpecificityRecognizes human mast cell tryptase, both alpha and beta isoforms. The antibody is an excellent marker for mast cells and does not bind to any other cell type in Immunohistochemistry. Species Crossreactivity: canine, feline, monkey and rat
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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