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You are here:Home » Antibodies » Abs to Matrix Metalloproteinases » Anti -Matrix Metalloproteinase 1 (MMP-1, Interstitial Collagenase 1) (BSA & Azide Free)

Anti -Matrix Metalloproteinase 1 (MMP-1, Interstitial Collagenase 1) (BSA & Azide Free)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IH
Matrix metalloproteinases (MMP) are proteolytic enzymes capable of degrading connective tissue components. Imbalanced secretion of certain MMP or disturbances in the differential control of MMP by tissue inhibitor of MMPs (TIMPs) are implicated in the invasive potential of malignant tumors. Interstitial collagenase (MMP-1), 72kD gelatinase A/type IV collagenase (MMP-2), and 92kD gelatinase B/type IV collagenase (MMP-9) dissolve extracellular matrix (ECM) and may initiate and promote angiogenesis.
Catalog #M2420-03X
MW of Antigen 52kD (unglycosylated) and 57kD (glycosylated) species of pro form of MMP-1 plus 42kD (unglycosylated) and 47kD (glycosylated) active form of MMP-1.
Epitope Middle region of MMP-1
Applications and Recommended DilutionsWestern Blotting (is better) (Ab 5-10ug/ml for 2 hrs at RT)
Immunohistochemistry (Formalin/paraffin) (Use Ab at 10-20ug/ml for 30 min at RT) * [Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.] The optimal dilution for a specific application should be determined by the investigator.
Positive Control Conditioned, serum-free medium from (TPA-treated) HFL-1 cells. Placenta. Bladder, breast, ovarian carcinoma.
Cellular Localization Cytoplasmic
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at-20°C or colder. Aliquots are stable for at least 12 months at-20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in 10mM PBS, pH 7.4. Also available with BSA and azide. See M2420-03
PurityPurified by Protein A affinity chromatography.
ImmunogenA synthetic peptide from the middle region of the human MMP-1
SpecificityRecognizes Matrix Metalloproteinase 1 (MMP-1, Interstitial Collagenase 1). Species Crossreactivity: human. Does not react with bovine.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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