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You are here:Home » Antibodies » Abs to Matrix Metalloproteinases » Anti -Matrix Metalloproteinase 1 (MMP-1, Fibroblast/Interstital Collagenase-1)

Anti -Matrix Metalloproteinase 1 (MMP-1, Fibroblast/Interstital Collagenase-1)

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Specifications

Clone Host Grade Applications
Monoclonal Rabbit Supernatant B IP IH IC FC
Human interstitial collagenase (matrix metalloproteinase-1, MMP-1), an enzyme whose only known physiologic substrate has heretofore been believed to be the extracellular matrix molecule, collagen. Data indicate that matrix metalloproteinase-1 displays an expanded substrate repertoire that supports the existence of a new interface between connective tissue turnover and serine proteinase inhibitors (1). It has been shown that the MMP-1 functions as a protease agonist of Protease-activated receptors (PAR1) cleaving the receptor at the proper site to generate PAR1-dependent Ca2+ signals and migration. These results demonstrate that MMP-1 in the stromal-tumor microenvironment can alter the behavior of cancer cells through PAR1 to promote cell migration and invasion (2). It has also been suggested that increased levels of MMP-1 due to tobacco smoking plays a major role in the aging process of skin since MMP-1 degrades collagen, which accounts for at least 70% of the dry weight of dermis. Significantly more MMP-1 has been detected in the skin of smokers than non-smokers whereas no difference was seen for the tissue inhibitor of metalloproteinases 1 (3).
Specificity: A synthetic peptide corresponding to residues on human MMP-1 was used as an immunogen.
Storage Conditions: Store at -20 °C. Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.
Recommended Dilutions:
Western Blot: 1:1000
Immunohistochemistry: 1:50-100
ICC: 1:100-250
FC: 1:20
Immunoprecipitation: 1:30
Catalog #M2420-15A
ApplicationsSuitable for use in Flow Cytometry, ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000
Immunohistochemistry: 1:50-100
ICC: 1:100-250
FC: 1:20
Immunoprecipitation: 1:30
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Manufactured incorporating RabMAb® technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
Clone TypeMonoclonal
IsotypeIgG
Clone No9E359
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in 50 mM Tris-Glycine, pH 7.4, 0.15 M sodium chloride, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
PuritySupernatant
ImmunogenA synthetic peptide corresponding to residues on human MMP-1
SpecificityRecognizes human MMP-1.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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