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You are here:Home » Antibodies » Abs to Matrix Metalloproteinases » Anti -MMP-2 (72kD Type IV Collagenase, 72kD Gelatinase, Gelatinase A, Matrix Metalloproteinase-2, TBE-1, MMP2, CLG4A)

Anti -MMP-2 (72kD Type IV Collagenase, 72kD Gelatinase, Gelatinase A, Matrix
Metalloproteinase-2, TBE-1, MMP2, CLG4A)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IH
MMPs are proteolytic enzymes capable of degrading connective tissue components. They have a common mode of activation, a conserved aa sequence in the putative metal binding-active site region, and inhibited by specific tissue inhibitors of metalloproteinases (TIMPs). MMPs and TIMPs play a significant role in regulating angiogenesis. MMP-2; also known as 72kD collagenase IV or gelatinase A is synthesized as a 631aa proenzyme, which is activated by cleavage of the first 80aa into an active form of 62kD. This antibody reacts with only the proform of MMP-2 and shows no cross-reaction with pro and active forms of MMP-9.
Catalog #M2420-50A
ApplicationsSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionsWestern Blot: 1ug/ml for 2 hours at RT
Immunohistochemistry (Formalin/paraffin): 1:50 for 30 minutes at RT. No special treatment is required for staining formalin/paraffin tissues.
Optimal dilutions to be determined by the researcher.
Positive ControlConditioned, serum-free medium from (TPA-treated) human large cell lymphoma U937 cells, placenta
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1
Clone NoSPM346
HostMouse
Concentration~0.1mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.05% sodium azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenSynthetic peptide corresponding to procollagenase IV at N-terminal. Cellular Localization: Cytoplasmic
SpecificityRecognizes MMP-2. Species Crossreactivity: human, mouse and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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