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You are here:Home » Antibodies » Antibodies-Matrix Metalloproteinases » Anti -Matrix Metalloproteinase 9 (MMP-9, Collagenase IV, Gelatinase B)

Anti -Matrix Metalloproteinase 9 (MMP-9, Collagenase IV, Gelatinase B)


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Clone Host Grade Applications
Monoclonal Mouse Purified B IH
All cells within tissues are surrounded by an extracellular matrix (ECM) giving the tissues shape and structure. The ECM is constantly being remodeled and constant communication is maintained between cells through this matrix. Secreted proteins, termed matrix metalloproteinases (MMPs), are involved in the modulation of cell matrix interactions. MMPs are Zn(2+) binding endopeptidases that degrade various components of the ECM. MMPs are enzymes implicated in normal and pathologic tissue remodeling processes, wound healing, angiogenesis, and tumor invasion. These enzymes are very potent when active, and are associated with extracellular space inhibitors called TIMPs (tissue inhibitors of matrix metalloproteinases). MMP9, also known as gelatinase B, is a secreted enzyme which degrades the interstitial collagens, types I, II, and III and is produced by normal alveolar macrophages and granulocytes.
Catalog #M2425-21
ApplicationsSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionsWestern Blot: 1:500. Detected MMP-9 on 10ug of human spleen lysates. Human spleen lysates were resolved by electrophoresis, transferred to PVDF membrane and probed with M2425-21 (1:500). Proteins were visualized using an IgG (HRP) goat anti-mouse and a chemiluminescence detection system.
Immunohistochemistry (Frozen, paraffin): 1-5ug/ml. PLP Fixation preferred. Will also work on formalin fixed tissues.
Optimal dilutions to be determined by the researcher.
Recommended Secondary AntibodiesI1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo I1904-06H IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
Clone No2Q521
FormSupplied as a liquid in 0.1M phosphate buffer, pH 7.0, 2% BSA (protease free).
PurityPurified by Protein A affinity chromatography.
ImmunogenSynthetic peptide corresponding to aa626-644 of human MMP-9, RSASEVDRMFPGVPLDTHD. Epitope: Near C-terminus.
SpecificityRecognizes human MMP-9. Recognizes precursor (92kD) and active (83kD) forms of human and guinea pig MMP-9. Does not react with the human MMP-1, 2, 3 or 13. Species Crossreactivity: guinea pig, rat and rabbit. Does not react with mouse, hamster, or bovine.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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