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You are here:Home » Antibodies » Abs to Matrix Metalloproteinases » Anti -Matrix Metalloproteinase 10 (MMP-10, Stromilysin 2, Transin 2) (BSA & Azide Free)

Anti -Matrix Metalloproteinase 10 (MMP-10, Stromilysin 2, Transin 2) (BSA & Azide Free)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IP
Matrix metalloproteinases (MMP) are proteolytic enzymes capable of degrading extracellular matrix. Stromelysin, a member of the matrix metalloproteinase family, demonstrates wide substrate specificity with the ability to degrade proteoglycan, fibronectin, laminin, casein, and the nonhelical region of collagen. The two forms of stromelysin (SL), types 1 (MMP-3) and 2 (MMP-10), share 82% sequence homology, but exhibit differences in cellular synthesis and inducibility by cytokines and growth factors in vitro.
Catalog #M2426-14X
ApplicationsSuitable for use in Western Blot and Immunoprecipitation. Other applications not tested.
Recommended DilutionWestern Blot: 1-2ug/ml for 2 hours at RT
Immunoprecipitation (Native verified): 2ug/mg protein lysate. Use Protein A
Optimal dilutions to be determined by the researcher.
Positive ControlConditioned, serum-free medium from (TPA-treated) human large cell lymphoma U937 cells. Placenta. Bladder, breast, and ovarian carcinomas.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2b
Clone No3F266
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.4. Also available with BSA and azide. See M2426-14
PurityPurified by Protein A affinity chromatography.
ImmunogenHuman recombinant MMP-10 (stromelysin-2 protein).
MW of Antigen~57kD (pro form) and ~44kD (active form) Cellular Localization: Cytoplasmic
SpecificityRecognizes pro (latent) and active forms of human MMP-10.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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