| Matrix metalloproteinases (MMP) are proteolytic enzymes capable of degrading extracellular matrix. Stromelysin, a member of the matrix metalloproteinase family, demonstrates wide substrate specificity with the ability to degrade proteoglycan, fibronectin, laminin, casein, and the nonhelical region of collagen. The two forms of stromelysin (SL), types 1 (MMP-3) and 2 (MMP-10), share 82% sequence homology, but exhibit differences in cellular synthesis and inducibility by cytokines and growth factors in vitro. |
|
| Catalog # | M2426-14X |
| Applications | Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. |
| Recommended Dilution | Western Blot: 1-2ug/ml for 2 hours at RT |
| Immunoprecipitation (Native verified): 2ug/mg protein lysate. Use Protein A |
| Optimal dilutions to be determined by the researcher. |
| Positive Control | Conditioned, serum-free medium from (TPA-treated) human large cell lymphoma U937 cells. Placenta. Bladder, breast, and ovarian carcinomas. |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG2b |
| Clone No | 3F266 |
| Host | Mouse |
| Source | Human |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in PBS, pH 7.4. Also available with BSA and azide. See M2426-14 |
| Purity | Purified by Protein A affinity chromatography. |
| Immunogen | Human recombinant MMP-10 (stromelysin-2 protein). |
| MW of Antigen | ~57kD (pro form) and ~44kD (active form) Cellular Localization: Cytoplasmic |
| Specificity | Recognizes pro (latent) and active forms of human MMP-10. |
|
| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
