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You are here:Home » Antibodies » Abs to Matrix Metalloproteinases » Anti -MMP-13 (Collagenase 3, Matrix Metalloproteinase-13, MMP13)

Anti -MMP-13 (Collagenase 3, Matrix Metalloproteinase-13, MMP13)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified IP IH
Human collagenase-3 (MMP13) is a recently identified member of the matrix metalloproteinase (MMP) family that is expressed in breast carcinomas and in articular cartilage from arthritic patients. The MMP13 gene has been isolated and characterized. This gene is composed of 10 exons and 9 introns and spans over 12.5kb. The overall organization of the collagenase-3 gene is similar to that of other MMP genes clustered chromosome 11q22.
Catalog #M2427-23C
ApplicationsSuitable for use in Immunoprecipitation and Immunohistochemistry. Other applications not tested.
Recommended DilutionImmunohistochemistry (Formalin fixed paraffin embedded): 1:20 for 30min at RT
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20min followed by cooling at RT for 20min
Immunoprecipitation: 2ug/mg protein lysate
Optimal dilutions to be determined by the researcher.
Positive ControlConditioned, serum-free medium from (TPA-treated) human chondrosarcoma SW1353 cells, placenta, bladder, breast, ovarian carcinomas
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone NoSPM292
HostMouse
Concentration~0.1mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.05% sodium azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenRecombinant corresponding to human MMP-13.
SpecificityRecognizes human MMP-13. Species Crossreactivity: mouse, and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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