Anti -MMP19 (MMP-19, Matrix Metalloproteinase 19, Matrix Metalloproteinase 18, MMP-18,
MMP18, Matrix Metalloproteinase RASI, RASI)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Goat |
Affinity Purified |
B IH |
|
| MP-19 (Matrix metalloprotease 19; also MMP-18 and MMP RASI) is a 55-59kD member of the peptidase M10A family of enzymes. It is widely expressed, being secreted by stratum basale keratinocytes, smooth muscle cells, epiphysial cartilage chondrocytes and monocytes/macrophages. MMP-19 has multiple substrates, including components of the basement membrane (type IV collagen; laminin; nidogen), fibronectin, aggrecan plus COMP, and IGFBP3, this latter cleavage resulting in the release of active IGF-I. Studies involving MMP-19 demonstrate an antiangiogenic function. This is attributable to the processing of plasminogen, generating angiostatin-like molecules, and the creation of an environment that promotes the ECM retention of soluble VEGF. Human proMMP-19 is 490aa in length. It contains an autocleavable 9kD propeptide aa19-97 plus a 411aa mature region aa98-508. The mature region contains a Zn catalytic region aa103-256 plus four hemopexin-like domains aa293-508. There are three additional potential isoforms. One shows an 88aa substitution for aa300-508, a second contains a12aa substitution for aa1-298, while a third possesses an alternative start site at Met80. Over aa229-508, human MMP-19 shares 76%aa identity with mouse MMP-19. | | | Catalog # | M2433-20E | | Applications | Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. | | Recommended Dilution | Western Blot: 1ug/ml | | Immunohistochemistry: 5-15ug/ml | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Goat | | Source | Human | | Concentration | ~0.1mg/ml after reconstitution | | Form | Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 0.2mg/ml. | | Purity | Purified by immunoaffinity chromatography. | | Immunogen | Recombinant corresponding to aa229-508 from human MMP-19 expressed in NSO cell line. | | Specificity | Recognizes human MMP-19. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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