Login

Forgot your password?
New User?
Remember me
banner banner

You are here:Home » Antibodies » Abs to Matrix Metalloproteinases » Anti -MMP19 (MMP-19, Matrix Metalloproteinase 19, Matrix Metalloproteinase 18, MMP-18, MMP18, Matrix Metalloproteinase RASI, RASI)

Anti -MMP19 (MMP-19, Matrix Metalloproteinase 19, Matrix Metalloproteinase 18, MMP-18,
MMP18, Matrix Metalloproteinase RASI, RASI)

Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified B IH
MP-19 (Matrix metalloprotease 19; also MMP-18 and MMP RASI) is a 55-59kD member of the peptidase M10A family of enzymes. It is widely expressed, being secreted by stratum basale keratinocytes, smooth muscle cells, epiphysial cartilage chondrocytes and monocytes/macrophages. MMP-19 has multiple substrates, including components of the basement membrane (type IV collagen; laminin; nidogen), fibronectin, aggrecan plus COMP, and IGFBP3, this latter cleavage resulting in the release of active IGF-I. Studies involving MMP-19 demonstrate an antiangiogenic function. This is attributable to the processing of plasminogen, generating angiostatin-like molecules, and the creation of an environment that promotes the ECM retention of soluble VEGF. Human proMMP-19 is 490aa in length. It contains an autocleavable 9kD propeptide aa19-97 plus a 411aa mature region aa98-508. The mature region contains a Zn catalytic region aa103-256 plus four hemopexin-like domains aa293-508. There are three additional potential isoforms. One shows an 88aa substitution for aa300-508, a second contains a12aa substitution for aa1-298, while a third possesses an alternative start site at Met80. Over aa229-508, human MMP-19 shares 76%aa identity with mouse MMP-19.
Catalog #M2433-20E
ApplicationsSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1ug/ml
Immunohistochemistry: 5-15ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceHuman
Concentration~0.1mg/ml after reconstitution
FormSupplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 0.2mg/ml.
PurityPurified by immunoaffinity chromatography.
ImmunogenRecombinant corresponding to aa229-508 from human MMP-19 expressed in NSO cell line.
SpecificityRecognizes human MMP-19.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


External Links