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M2450-39B Goat Anti-MAD3 (Max Dimerization Protein 3, Max Dimerizer 3, Class C Basic Helix-loop-helix Protein 13, bHLHc13, Max-associated Protein 3, Max-interacting Transcriptional Repressor MAD3, Myx, MXD3, BHLHC13)

Specifications
References
Clone Type
Polyclonal
Host
Goat
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E
Crossreactivity
Hu
Accession #
NP_112590.1
Gene ID
83463
Shipping Temp
Blue Ice
Storage Temp
-20°C

Transcriptional repressor. Binds with MAX to form a sequence-specific DNA-binding protein complex which recognizes the core sequence 5'-CAC[GA]TG-3'. Antagonizes MYC transcriptional activity by competing for MAX and suppresses MYC dependent cell transformation.

Applications
Suitable for use in ELISA. Other applications not tested.
Recommended Dilution
ELISA: 1:4000 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to C-QEHSYSHGGGAWL, from human MAD3, at C-terminal (NP_112590.1).
Form
Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human MAD3.
References
1. Hurlin PJ, Queva C, Koskinen PJ, Steingrimsson E, Ayer DE, Copeland NG, Jenkins NA, Eisenman RN. |Mad3 and Mad4: novel Max-interacting transcriptional repressors that suppress c-myc dependent transformation and are expressed during neural and epidermal differentiation. EMBO J. 1995 Nov 15;14(22):5646-59.
USBio References
No references available
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