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You are here:Home » Antibodies » Abs to Transcription Factors, Zinc (Ring) Finger » Anti -MIZF (MBD2 Interacting Zinc Finger Protein, Methyl CpG Binding Protein 2 Interacting Zinc Finger Protein, MBD2 (Methyl-CpG-Binding Protein)-Interacting Zinc Finger Protein)

Anti -MIZF (MBD2 Interacting Zinc Finger Protein, Methyl CpG Binding Protein 2 Interacting
Zinc Finger Protein, MBD2 (Methyl-CpG-Binding Protein)-Interacting Zinc Finger Protein)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Serum B
DNA methylation, or the addition of methyl groups to cytosine bases in the dinucleotide CpG, is imperative to proper development and regulates gene expression. The methylation pattern involves the enzymatic processes of methylation and demethylation. The demethylation enzyme was recently found to be a mammalian protein, which exhibits demethylase activity associated to a methyl-CpG-binding domain (MBD) (1). The enzyme is able to revert methylated cytosine bases to cytosines within the particular dinucleotide sequence mdCpdG by catalyzing the cleavage of the methyl group as methanol. MeCP2 and MBD1 (PCM1) are first found to repress transcription by binding specifically to methylated DNA (2). MBD2 and MBD4 were later found to co-localize with foci of heavily methylated satellite DNA and believed to mediate the biological functions of the methylation signal. Surprisingly, MBD3 does not bind methylated DNA both in vivo and in vitro. MBD1, MBD2, MBD3, and MBD4 are found to be expressed in somatic tissues, but the expression of MBD1 and MBD2 is reduced or absent in embryonic stem cells, which are known to be deficient in MeCP1 activity. Recently, using MBD2 as bait in two-hybrid screening, a novel zinc finger protein, called MIZF has been identified (3). This MBD2-binding zinc finger (MIZF) protein represses transcription by associating with MBD2 and a histone deacetylase complex. It is ubiquitously expressed in all human tissues.
Catalog #M2471
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1:500 detects a band of ~64kD.
Optimal dilutions to be determined by the researcher.
Positive Control MOLT-4 cell lysate
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in 0.02% sodium azide.
PuritySerum
ImmunogenSynthetic peptides corresponding to aa residues, 180-194, 331-346, and 371-388 of human MIZF.
SpecificityRecognizes human MBD2-binding zinc finger (MIZF).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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