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You are here:Home » Antibodies » Abs to DNA Replication, Repair » Anti -MBD4 (Methyl-CpG-binding Domain Protein 4, Methyl-CpG-binding Endonuclease 1, Methyl-CpG-binding Protein MBD4, Mismatch-specific DNA N-glycosylase, MED1)

Anti -MBD4 (Methyl-CpG-binding Domain Protein 4, Methyl-CpG-binding Endonuclease 1,
Methyl-CpG-binding Protein MBD4, Mismatch-specific DNA N-glycosylase, MED1)

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B
DNA methylation, or the addition of methyl groups to cytosine bases in the dinucleotide CpG, is imperative to proper development and regulates gene expression. The methylation pattern involves the enzymatic processes of methylation and demethylation. The demethylation enzyme was recently found to be a mammalian protein, which exhibits demethylase activity associated to a methyl-CpG-binding domain (MBD) (1). The enzyme is able to revert methylated cytosine bases to cytosines within the particular dinucleotide sequence mdCpdG by catalyzing the cleaving of the methyl group as methanol. MeCP2 and MBD1 (PCM1) are first found to repress transcription by binding specifically to methylated DNA (2). MBD2 and MBD4 (also known as MED1) were later found to colocalize with foci of heavily methylated satellite DNA and believed to mediate the biological functions of the methylation signal. Surprisingly, MBD3 does not bind methylated DNA both in vivo and in vitro. MBD1, MBD2, MBD3, and MBD4 are found to be expressed in somatic tissues, but the expression of MBD1 and MBD2 is reduced or absent in embryonic stem cells, which are known to be deficient in MeCP1 activity. MBD4 have homology to bacterial base excision repair DNA N-glycosylases/lyases (3). In some microsatellite unstable tumors MBD4 is mutated at an exonic polynucleotide tract (4).
Catalog #M2480
ApplicationsCan be used for detection of MBD4 by Western blot. A protein of 64kD can be detected in HL60 cells. It has not been tested in other applications. Western blot analysis of MBD4 in HL-60 cell lysate with anti-MBD4 pcAb. A protein band of approximate molecular weight of 64kD was detected.
Recommended DilutionWestern blot: 2ug/ml.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, 0.02% sodium azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenSynthetic peptides corresponding to amino acids 268-282 and 337-352 of human MBD4.
SpecificityRecognizes human MBD4 (Methyl CpG Binding Protein 4)
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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