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You are here:Home » Molecular Biology » Growth Factors-MCAF » MCAF, Recombinant, Human (MCP-1, Macrophage/Monocyte Chemotactic and Activating Factor)

MCAF, Recombinant, Human (MCP-1, Macrophage/Monocyte Chemotactic and Activating Factor)

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Specifications

Recombinant human MCP-1 is a non-glycosylated, polypeptide chain containing 76 amino acids.
Catalog #M2690-01A
Endotoxin< 0.01ng/ug or < 0.1EU/ug as per LAL
Biological ActivityMCP-1 is fully biologically active when compared to standard. The specific activity is determined by the ability of MCP-1 to chemoattract human Monocytes using a concentration of 5-20ng/ml.
AA SequenceQPDAINAPVT CCYNFTNRKI SVQRLASYRR ITSSKCPKEA VIFKTIVAKE ICADPKQKWV QDSMDHLDKQ TQTPKT
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O to concentration of 0.1-1mg/ml with carrier protein (1% HSA or BSA). Aliquot and store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight8.7kD
SourceRecombinant, human, produced in E. coli
Purity98% as determined by RP-HPLC; Reducing and non-reducing SDS-PAGE.
FormSupplied as a lyophilized powder. Reconstitute with sterile ddH2O to 0.1-1mg/ml, which can be further diluted into other aqueous solutions. For long term storage, it is recommended to add a carrier protein (0.1% HSA or BSA).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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