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MCAF, Recombinant, Rat (MCP-1, Macrophage/Monocyte Chemotactic and Activating Factor)
MCAF, Recombinant, Rat (MCP-1, Macrophage/Monocyte Chemotactic and Activating Factor)
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| Chemokine (C-C motif) ligand 2 (CCL2) is a small cytokine belonging to the CC chemokine family that is also known as monocyte chemotactic protein-1 (MCP-1). It is found at the site of tooth eruption and bone degradation. In the bone, CCL2 is expressed by mature osteoclasts and osteoblasts and is under the control of nuclear factor kappa B (NFkappaB). CCL2 recruits immune cells, such as monocytes, to sites of tissue injury and infection. This chemokine is produced as a protein precursor containing signal peptide of 23aa and a mature peptide of 76aa. It is a monomeric polypeptide, with a molecular weightof approximately 13kD. As with many other CC chemokines, CCL2 is located on chromosome 17 in humans. The cell surface receptors that bind CCL2 are CCR2 and CCR5 | | | Catalog # | M2690-15B | | Recombinant Rat MCP-1 is a non-glycosylated, Polypeptide chain containing 125aa and having a molecular mass of 14.09kD. | | Sequence | The sequence of the first five N-terminal amino acids was determined and was found to be Gln-Pro-Asp-Ala-Val. | | Biological Activity | MCP-1 is fully biologically active when compared to standard. The specific activity as determined by the ability of Rat MCP-1 to chemoattract human peripheral blood at 10-100ng/ml. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, add 0.1% BSA or HSA, aliquot to avoid repeated freezing and thawing and freeze at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 12 months. | | Molecular Weight | 14.09kD | | Source | E. coli | | Purity | 95% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified. | | Concentration | ~0.1mg/ml | | Form | Supplied as a sterile filtered liquid in 20mM citrate, pH 3.5. No stabilizers added. | | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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