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You are here:Home » BioSeparation? » BioSep-Assay Detection, Tags » mCherry Fluorescent Protein, Recombinant

mCherry Fluorescent Protein, Recombinant

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Specifications

mCherry is the second generation monomeric red fluorescent protein that have improved brightness and photostability. The recombinant mCherry is expressed and purified from transformed E. coli using a method that ensures high purity and maximal fluorescence intensity.
Catalog #M2690-20
The protein is a 28.8kD monomer with 256 amino acids, pI 6.23. Ex.= 587 nm (540-590 nm); Em.= 610 nm (550-650 nm). The protein is engineered with 6xHis-tag on the N-terminus, which can be used for detection with anti-His-Tag antibody or protein purification/removal by using Ni++ beads.
Amino Acid SequenceMGSSHHHHHHSSGLVPRGSHMVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAEGRHSTGGMDELYK
ApplicationsSuitable for use as standard/positive control for SDS-PAGE and for Western Blot analysis of mCherry-transfected cells. Suitable for use in labeling proteins or antibodies. Also suitable for use in calibration of fluorometers and flow cytometers, and fluorescence microscope. mCherry protein can be microinjected into cells and tissues and is also ideal for fuison tag applications. Can be used for triple labeling with EGFP, CFP, YFP and other dyes. The 6xHis-Tag on the N-terminal can be used in Western Blot detection with 6xHis-Tag antibodies. The 6xHis-Tag can also be used in removal or purification of the mCherry protein. Other applications not tested.
Storage and StabilityLyophilized powder may be stored at -70°C. Stable for 12 months at -70°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -70°C. Aliquot to avoid repeated freezing and thawing and store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS NumberN/A
Molecular Weight28.8kD
SourceE. coli
Purity97% (SDS-PAGE and HPLC). Endotoxin: 0.1ng/ug
FormSupplied as freeze dried powder. Reconstitute with 100ul sterile ddH2O.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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