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You are here:Home » Antibodies » Abs to DNA Replication, Repair » Anti -MCM2 (Mini Chromosome Maintenance Protein 2, MCM2 Minichromosomal Maintenance Deficient 2 Mitotin, BM28, Cyclin-like 1, CCNL1, Cell Devision Cycle-like 1, CDC like-1, CDCL1, cdc19, D3S3194, DNA Replication Licensing Factor MCM2, KIAA0030, MGC10606, Mitoti

Anti -MCM2 (Mini Chromosome Maintenance Protein 2, MCM2 Minichromosomal Maintenance
Deficient 2 Mitotin, BM28, Cyclin-like 1, CCNL1, Cell Devision Cycle-like 1, CDC like-1,
CDCL1, cdc19, D3S3194, DNA Replication Licensing Factor MCM2, KIAA0030, MGC10606, Mitoti

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IP
MCM2 (mini chromosome maintenance protein 2) is a putative DNA replicative helicase and member of the MCM family, critical during the initiation of DNA replication.
During the G1 phase of the cell cycle the MCM proteins, part of the pre-replicative complex which assembles at replication origins, undergo phosphorylation by kinases including cyclin dependent kinase (CDK) and Cdc7/Dbf4 (DDK). Studies have shown that the phosphorylation of MCM2 occurs on multiple sites, namely Ser27, Ser41, Ser53, Ser108 and Ser139 and that phosphorylation results in a conformational change of the complex and activation of the helicase activity.
Catalog #M2749-29
ApplicationsSuitable for use in Immunoprecipitation and Western Blot. Other applications not tested.
Recommended DilutionImmunoprecipitation: 2-5ug/mg lysate
Western Blot: 1:1,000-1:10,000. Detects a band of approximately 120-130kD in HeLa RIPA extracts.
Optimal dilutions to be determined by the researcher.
Control Peptide See M2749-29A
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in Tris-citrate/phosphate, 0.09% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide corresponding to an epitope within amino acid residues 1-50 of human MCM2 (NP_004517.2).
SpecificityRecognizes human MCM2 (mini chromosome maintenance protein 2) nuclear protein. Species Crossreactivity: Xenopus. Expected to crossreact with mouse based on sequence homology.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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