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You are here:Home » Antibodies » Abs to DNA Replication, Repair » Anti -MCM2 (Mini Chromosome Maintenance Protein 2, MCM2 Minichromosomal Maintenance Deficient 2 Mitotin, BM28, Cyclin-like 1, CCNL1, Cell Devision Cycle-like 1, CDC like-1, CDCL1, cdc19, D3S3194, DNA Replication Licensing Factor MCM2, KIAA0030, MGC10606, Mitoti

Anti -MCM2 (Mini Chromosome Maintenance Protein 2, MCM2 Minichromosomal Maintenance
Deficient 2 Mitotin, BM28, Cyclin-like 1, CCNL1, Cell Devision Cycle-like 1, CDC like-1,
CDCL1, cdc19, D3S3194, DNA Replication Licensing Factor MCM2, KIAA0030, MGC10606, Mitoti

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IH
The mini-chromosome maintenance (MCM) family of proteins, including MCM2, MCM3, MCM4 (Cdc21), MCM5(Cdc46), MCM6(Mis5) and MCM7(Cdc47), are regulators of DNA replication that act to ensure replication occurs only once in the cell cycle. Expression of MCM proteins increases during cell growth, peaking at G1 to S phase. MCM2/MCM4 complexes function as substrates for Cdc2/cyclin B in vitro.
Catalog #M2749-29F
ApplicationsSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionImmunohistochemistry (Formalin fixed paraffin embedded): 1:100 for 30min at RT
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10min followed by cooling at RT for 20min.
Optimal dilutions to be determined by the researcher.
Positive ControlHeLa cells, breast carcinoma
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
HostRabbit
SourceHuman
Concentration~0.1mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.05% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide corresponding to the N-terminus of human MCM2.
SpecificityRecognizes human MCM2. Species Crossreactivity: mouse and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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