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You are here:Home » Antibodies » Abs to DNA Replication, Repair » Anti -MCM2 (Minichromosome maintenance complex component 2, BM28, CCNL1, cdc19, CDCL1, D3S3194, DNA replication licensing factor MCM2, KIAA0030, MGC10606, Minichromosome maintenance protein 2 homolog, MITOTIN, Nuclear protein BM28)

Anti -MCM2 (Minichromosome maintenance complex component 2, BM28, CCNL1, cdc19, CDCL1,
D3S3194, DNA replication licensing factor MCM2, KIAA0030, MGC10606, Minichromosome
maintenance protein 2 homolog, MITOTIN, Nuclear protein BM28)

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity purified B
MCM2 (Minichromosome maintenance protein 2; also BM28) is a member of the MCM family of proteins. Although its predicted MW is 102kD, it runs anomalously at 125kD in SDS-PAGE. MCM2 forms a large, 450-600kD prereplication complex with MCM3 through 7 plus HBO1, and associates with the ORC to initiate DNA synthesis. Human MCM2 is 904 amino acids (aa) in length (Accession # P49736). It contains one C4-type zinc finger region (aa329-355) and a P-loop NTPase region (aa485-659) and at least 12 Ser/Thr phosphorylation sites in the N-terminal one-third of the molecule. MCM2 has several potential isoforms. There is an alternate start site at Met10 with or without a 47 aa substitution for aa 109-157, a deletion of aa 31-126, a Gly substitution for aa 113-132 coupled with a 69 aa insertion after Lys476, and a seven aa substitution for aa1-137. Over aa143-296, human MCM2 shares 99% aa identity with mouse MCM2.
Catalog #M2749-30Z
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot:1ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute by adding sterile 40-50% glycerol, PBS, 0.02% sodium azide. Aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceHuman
ConcentrationAs reported
FormSupplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 100ul sterile 40-50% glycerol, PBS, 0.02% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenRecombinant corresponding to aa143-296 of human MCM2 (E. coli).
SpecificityRecognizes human MCM2at 102kD.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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