Anti -MCM2 (Minichromosome maintenance complex component 2, BM28, CCNL1, cdc19, CDCL1,
D3S3194, DNA replication licensing factor MCM2, KIAA0030, MGC10606, Minichromosome
maintenance protein 2 homolog, MITOTIN, Nuclear protein BM28)
Pricing
For pricing information, USA customers sign in. Outside USA? Please contact your distributor for pricing. Specifications
| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Goat |
Affinity purified |
B |
|
| MCM2 (Minichromosome maintenance protein 2; also BM28) is a member of the MCM family of proteins. Although its predicted MW is 102kD, it runs anomalously at 125kD in SDS-PAGE. MCM2 forms a large, 450-600kD prereplication complex with MCM3 through 7 plus HBO1, and associates with the ORC to initiate DNA synthesis. Human MCM2 is 904 amino acids (aa) in length (Accession # P49736). It contains one C4-type zinc finger region (aa329-355) and a P-loop NTPase region (aa485-659) and at least 12 Ser/Thr phosphorylation sites in the N-terminal one-third of the molecule. MCM2 has several potential isoforms. There is an alternate start site at Met10 with or without a 47 aa substitution for aa 109-157, a deletion of aa 31-126, a Gly substitution for aa 113-132 coupled with a 69 aa insertion after Lys476, and a seven aa substitution for aa1-137. Over aa143-296, human MCM2 shares 99% aa identity with mouse MCM2. | | | Catalog # | M2749-30Z | | Applications | Suitable for use in Western Blot. Other applications not tested. | | Recommended Dilution | Western Blot:1ug/ml | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute by adding sterile 40-50% glycerol, PBS, 0.02% sodium azide. Aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Goat | | Source | Human | | Concentration | As reported | | Form | Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 100ul sterile 40-50% glycerol, PBS, 0.02% sodium azide. | | Purity | Purified by immunoaffinity chromatography. | | Immunogen | Recombinant corresponding to aa143-296 of human MCM2 (E. coli). | | Specificity | Recognizes human MCM2at 102kD. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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Download Specifications for Anti -MCM2 (Minichromosome maintenance complex component 2, BM28, CCNL1, cdc19, CDCL1, D3S3194, DNA replication licensing factor MCM2, KIAA0030, MGC10606, Minichromosome maintenance protein 2 homolog, MITOTIN, Nuclear protein BM28) |
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