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You are here:Home » Antibodies » Abs to Enzymes, Polymerase » Anti -MCM3 (Cervical Cancer Proto-oncogene 5, DNA Polymerase alpha Holoenzyme Associated Protein P1, DNA Replication Licensing Factor MCM3, HCC5, hRlf beta Subunit, Human Cervical Cancer Proto-oncogene 5, MGC1157, Minichromosome Maintenance Deficient 3, Minichr

Anti -MCM3 (Cervical Cancer Proto-oncogene 5, DNA Polymerase alpha Holoenzyme Associated
Protein P1, DNA Replication Licensing Factor MCM3, HCC5, hRlf beta Subunit, Human Cervical
Cancer Proto-oncogene 5, MGC1157, Minichromosome Maintenance Deficient 3, Minichr

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IF
The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein is a subunit of the protein complex that consists of MCM2-7. It has been shown to interact directly with MCM5/CDC46. This protein also interacts with, and thus is acetlyated by MCM3AP, a chromatin-associated acetyltransferase. The acetylation of this protein inhibits the initiation of DNA replication and cell cycle progression.
Catalog #M2749-31J
ApplicationsSuitable for use in ELISA, Immunofluorescence and Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1:500-1:1000
ELISA: 1ug/ml-3ng/ml
Optimal dilutions to be determined by the researcher.
HybridomaSp2/0 myeloma cells with spleen cells from Balb/c mice.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No10B2435
HostMouse
SourceHuman
ConcentrationAs reported
FormSupplied as a liquid in PBS, pH 7.2. No preservative added (Azide free).
PurityPurified by Protein A affinity chromatography.
ImmunogenRecombinant protein corresponding to aa699-809 of human MCM3.
SpecificityRecognizes human MCM3.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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