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You are here:Home » Antibodies » Abs to DNA Replication, Repair » Anti -MCM5 (Mini Chromosome Maintenance Protein 5, CDC46, CDC46 Homolog, Cell Division Cycle 46, DNA Replication Licensing Factor MCM5, MGC5315, Minichromosome Maintenance Deficient (S. cerevisiae) 5, Minichromosome Maintenance Deficient Protein 5, P1-CDC46)

Anti -MCM5 (Mini Chromosome Maintenance Protein 5, CDC46, CDC46 Homolog, Cell Division
Cycle 46, DNA Replication Licensing Factor MCM5, MGC5315, Minichromosome Maintenance
Deficient (S. cerevisiae) 5, Minichromosome Maintenance Deficient Protein 5, P1-CDC46)

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IP
During the G1 phase of the cell cycle the MCM proteins, part of the pre-replicative complex which assembles at replication origins, undergo phosphorylation by kinases including cyclin dependent kinase (CDK) and Cdc7/Dbf4 (DDK), although the latter does not appear to phosphorylate MCM5 in vitro. The level of MCM5 is upregulated during the G1/S cell cycle phase and studies have shown a correlation between increasing levels of MCM5 and increased Stat1-mediated transcription activation in response to IFNgamma.
Catalog #M2749-35F
ApplicationsSuitable for use in Immunoprecipitation and Western Blot. Other applications not tested.
Recommended DilutionImmunoprecipitation: 2-4ug/mg
Western Blot: 1:5,000-1:20,000
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in tris-citrate/phosphate, 0.09% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide corresponding to an epitope within amino acid residues 1-50 of human MCM5 (NP_006730.2).
SpecificityRecognizes the nuclear protein of human MCM5 (mini chromosome maintenance protein 5), a putative DNA replicative helicase and member of the MCM family. Species Crossreactivity: mouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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