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Abs to DNA Replication, Repair
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Anti -MCM5 (Mini Chromosome Maintenance Protein 5, CDC46, CDC46 Homolog, Cell Division Cycle 46, DNA Replication Licensing Factor MCM5, MGC5315, Minichromosome Maintenance Deficient (S. cerevisiae) 5, Minichromosome Maintenance Deficient Protein 5, P1-CDC46)
Anti -MCM5 (Mini Chromosome Maintenance Protein 5, CDC46, CDC46 Homolog, Cell Division
Cycle 46, DNA Replication Licensing Factor MCM5, MGC5315, Minichromosome Maintenance
Deficient (S. cerevisiae) 5, Minichromosome Maintenance Deficient Protein 5, P1-CDC46)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Ascites |
IH |
|
| Minichromosome maintenance (MCM) family (MCM2-7) plays roles in the initiation and elongation of DNA replication. In eukaryotes, the MCM complex consists of six essential proteins (MCM2-7) and each protein contain conserved C-terminal helicase domains and divergent N-terminal sequences. The MCM proteins are components of the pre-replicative complex that assembles on replication origins before S phase. MCM5 encodes a member of the MCM2-7 protein family consists of 732 residues and involved in the initiation and elongation of DNA replication. The sequences of all MCM5 homologues from yeast to human are highly conserved and suggest that their functions are also conserved. The protein is structurally similar to the cell division cycle 46 (CDC46) protein form S. cerevisiae, a protein involved in ther initiation of DNA replication. The subcellular localization of MCM5 has been shown to be under cell cycle control, it appears in the nucleus at late M phase and persists in the nucleus until the G1/S transition. Northern and Western blot analyses revealed that the expression of MCM5 and its transcriptional regulator, E2F1, is negatively regulated by p53. | | | Catalog # | M2749-35H | | Applications | Suitable for use in Immunohistochemistry. Other applications not tested. | | Recommended Dilution | Immunohistochemistry (formalin fixed paraffin embedded): 1:25-1:50 | | Optimal dilutions to be determined by the researcher. | | Positive Control | Cervical carcinoma | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG2b | | Clone No | 11E273 | | Host | Mouse | | Source | Human | | Concentration | Not determined. | | Form | Supplied as a liquid, 0.05% sodium azide. | | Purity | Ascites | | Immunogen | Bacterially expressed human MCM5 protein. Cellular Localization: Nuclear. | | Specificity | Recognizes human MCM 5. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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