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You are here:Home » Antibodies » Abs to DNA Replication, Repair » Anti -MCM5 (Mini Chromosome Maintenance Protein 5, CDC46, CDC46 Homolog, Cell Division Cycle 46, DNA Replication Licensing Factor MCM5, MGC5315, Minichromosome Maintenance Deficient (S. cerevisiae) 5, Minichromosome Maintenance Deficient Protein 5, P1-CDC46)

Anti -MCM5 (Mini Chromosome Maintenance Protein 5, CDC46, CDC46 Homolog, Cell Division
Cycle 46, DNA Replication Licensing Factor MCM5, MGC5315, Minichromosome Maintenance
Deficient (S. cerevisiae) 5, Minichromosome Maintenance Deficient Protein 5, P1-CDC46)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IH
During the G1 phase of the cell cycle the MCM proteins, part of the pre-replicative complex which assembles at replication origins, undergo phosphorylation by kinases including cyclin dependent kinase (CDK) and Cdc7/Dbf4 (DDK), although the latter does not appear to phosphorylate MCM5 in vitro. The level of MCM5 is upregulated during the G1/S cell cycle phase and studies have shown a correlation between increasing levels of MCM5 and increased Stat1-mediated transcription activation in response to IFNgamma.
Catalog #M2749-35J
ApplicationsSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionImmunohistochemistry (Formalin fixed paraffin embedded): 1:50
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceMouse
ConcentrationNot determined
FormSupplied as a liquid in PBS, pH 7.2, 0.09% sodium azide, 50% glycerol.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide corresponding to KEVADEVTRPRPSGE mouse MCM5 (KLH).
SpecificityRecognizes mouse MCM5. Species sequence homology: human
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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