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You are here:Home » Antibodies » Abs to DNA Replication, Repair » Anti -MCM7 (Minichromosome Maintenance Protein 7, CDABP0042, CDC47, CDC47 Homolog, DNA Replication Licensing Factor MCM7, Homolog of S. cerevisiae Cdc47, MCM7 Minichromosome Maintenance Deficient 7, Minichromosome Maintainence 7, P1.1-MCM3, P1CDC47, P85MCM, PNA

Anti -MCM7 (Minichromosome Maintenance Protein 7, CDABP0042, CDC47, CDC47 Homolog, DNA
Replication Licensing Factor MCM7, Homolog of S. cerevisiae Cdc47, MCM7 Minichromosome
Maintenance Deficient 7, Minichromosome Maintainence 7, P1.1-MCM3, P1CDC47, P85MCM, PNA

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IP
The minichromosome maintenance (MCM7) helicase complex functions to initiate and elongate replication forks. Cell cycle checkpoint signaling pathways regulate DNA replication to maintain genomic stability (1). MCM7 is a putative human homologue of yeast CDC47 and a member of the MCM protein family, which has been implicated in the regulatory machinery causing DNA to replicate only once in the S phase. Findings indicate that MCM7 protein together with other MCM proteins participates in the regulation of mammalian DNA replication (2). In HeLa cells, depletion of MCM7 with small-interfering RNA suppressed ultraviolet (UV) light- or aphidicolin-induced hChk1 phosphorylation, and abolished UV-induced S-phase checkpoint activation. These results demonstrate that MCM7 plays a direct role in the transmission of DNA damage signals from active replication forks to the S-phase checkpoint machinery in human cells (3).
Applications:
Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested.
Catalog #M2749-37D
Recommended DilutionWestern Blot: 1:5000
Immunoprecipitation: 1:20
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in 50mM Tris-Glycine, pH 7.4, 0.18M sodium chloride, 40% glycerol, 0.25% sodium azide, 0.25mM EDTA, 25mM Citric Acid, 0.5% BSA.
PurityPurified by Immunoaffinity chromatography.
ImmunogenSynthetic peptide corresponding to residues near the N-terminus of human MCM7.
SpecificityRecognizes human MCM7. Species Crossreactivity: Rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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