Anti -MCM7 (Minichromosome Maintenance Protein 7, CDABP0042, CDC47, CDC47 Homolog, DNA
Replication Licensing Factor MCM7, Homolog of S. cerevisiae Cdc47, MCM7 Minichromosome
Maintenance Deficient 7, Minichromosome Maintainence 7, P1.1-MCM3, P1CDC47, P85MCM, PNA
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Affinity Purified |
B IP |
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| The minichromosome maintenance (MCM7) helicase complex functions to initiate and elongate replication forks. Cell cycle checkpoint signaling pathways regulate DNA replication to maintain genomic stability (1). MCM7 is a putative human homologue of yeast CDC47 and a member of the MCM protein family, which has been implicated in the regulatory machinery causing DNA to replicate only once in the S phase. Findings indicate that MCM7 protein together with other MCM proteins participates in the regulation of mammalian DNA replication (2). In HeLa cells, depletion of MCM7 with small-interfering RNA suppressed ultraviolet (UV) light- or aphidicolin-induced hChk1 phosphorylation, and abolished UV-induced S-phase checkpoint activation. These results demonstrate that MCM7 plays a direct role in the transmission of DNA damage signals from active replication forks to the S-phase checkpoint machinery in human cells (3). | | | | | | Applications: | | | Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. | | | Catalog # | M2749-37D | | Recommended Dilution | Western Blot: 1:5000 | | Immunoprecipitation: 1:20 | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Rabbit | | Source | Human | | Concentration | As reported | | Form | Supplied as a liquid in 50mM Tris-Glycine, pH 7.4, 0.18M sodium chloride, 40% glycerol, 0.25% sodium azide, 0.25mM EDTA, 25mM Citric Acid, 0.5% BSA. | | Purity | Purified by Immunoaffinity chromatography. | | Immunogen | Synthetic peptide corresponding to residues near the N-terminus of human MCM7. | | Specificity | Recognizes human MCM7. Species Crossreactivity: Rat. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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Download Specifications for Anti -MCM7 (Minichromosome Maintenance Protein 7, CDABP0042, CDC47, CDC47 Homolog, DNA Replication Licensing Factor MCM7, Homolog of S. cerevisiae Cdc47, MCM7 Minichromosome Maintenance Deficient 7, Minichromosome Maintainence 7, P1.1-MCM3, P1CDC47, P85MCM, PNA |
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