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You are here:Home » Molecular Biology » Growth Factors-MCP » MCP2, Recombinant, Human (Macrophage/Monocyte Chemotactic Protein 2, Chemokine C-C Motif Ligand 8, CCL8, HC14, Monocyte Chemotactic Protein 2, Small Inducible Cytokine A8 Precursor, SCYA8, SCYA10)

MCP2, Recombinant, Human (Macrophage/Monocyte Chemotactic Protein 2, Chemokine C-C Motif
Ligand 8, CCL8, HC14, Monocyte Chemotactic Protein 2, Small Inducible Cytokine A8
Precursor, SCYA8, SCYA10)

Pricing

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Specifications

Recombinant Human MCP-2 is a non-glycosylated, polypeptide chain containing 76 amino acids and having a molecular mass of 8904 Dalton.
Catalog #M2750-11A
SequenceThe sequence of the first five N-terminal amino acids was determined and was found to be Gln-Pro-Asp-Ser-Val.
Dimers and Aggregates 1% as determined by silver-stained SDS-PAGE gel analysis.
Biological ActivityMCP-2 is fully biologically active when compared to standard. The specific activity as determined by the ability of MCP-2 to chemoattract human peripheral blood at 10-100ng/ml.
Endotoxin 0.1ng/ug (IEU/ug) of Murine Monocyte Chemotactic Protein-2.
Protein ContentProtein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280nm.
2. Analysis by RP-HPLC, using a calibrated solution of Human MCP-2 as a Reference Standard.
ReconstitutionReconstitute the lyophilized MCP-2 in sterile 18MO-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile dH2O and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
SourceE. coli
Purity95% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.
Concentration~0.1mg/ml
FormSupplied as a lyophilized powder. No additives.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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