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You are here:Home » Antibodies » Abs to DEAD Box Proteins » Anti -MDA5 (Melanoma Differentiation-Associated Protein 5, Interferon-Induced with Helicase C Domain Protein 1, IFIH1)

Anti -MDA5 (Melanoma Differentiation-Associated Protein 5, Interferon-Induced with
Helicase C Domain Protein 1, IFIH1)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B
The innate immune system detects viral infection by recognizing various viral components and triggers antiviral responses (1,2). Like the toll-like receptor 3 (TLR3), the melanoma differentiation-associated protein 5 (MDA5) recognizes double-stranded (ds) RNA, a molecular pattern associated with viral infection. MDA5, a member of the DEAD/DEAH-box RNA helicase family (3), consists of an amino-terminal caspase recruitment domain (CARD) and a carboxyl-terminal RNA helicase domain similar to that of the related protein RIG-1. When stimulated by dsRNA, MDA5 recruits the adaptor protein VISA and ultimately causes the activation of IRF-3 and NF- B (4). MDA5 and RIG-1 recognize different types of dsRNA, with MDA5 recognizing poly (I:C). MDA5-null mice were highly susceptible to infection with picornaviruses, which possess such sequences, demonstrating the importance of MDA5 in innate immunity (5).
Catalog #M2772-75
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1-2ug/ml.
Optimal dilutions to be determined by the researcher.
Recommended Control PeptideM2772-75A: MDA5, Human, Control Peptide (Melanoma Differentiation-Associated Protein 5, Interferon-Induced with Helicase C Domain Protein 1, IFIH1)
Recommended Positive ControlDaudi cell lysate
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~1mg/ml.
FormSupplied as a liquid in PBS, 0.02% sodium azide.
PurityPurified by immunoaffinity chromatography.
Immunogen16 amino acid peptide from near the center of human MDA5 (GenBank accession no. NP_071451)
SpecificityRecognizes human MDA5. Species Crossreactivity: Mouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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