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Anti -MDA5, CT (Melanoma Differentiation-Associated Protein 5, Interferon-Induced with Helicase C Domain Protein 1, IFIH1)
Anti -MDA5, CT (Melanoma Differentiation-Associated Protein 5, Interferon-Induced with
Helicase C Domain Protein 1, IFIH1)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Affinity Purified |
B |
|
| The innate immune system detects viral infection by recognizing various viral components and triggers antiviral responses (1,2). Like the toll-like receptor 3 (TLR3), the melanoma differentiation-associated protein 5 (MDA5) recognizes double-stranded (ds) RNA, a molecular pattern associated with viral infection. MDA5, a member of the DEAD/DEAH-box RNA helicase family (3), consists of an amino-terminal caspase recruitment domain (CARD) and a carboxyl-terminal RNA helicase domain similar to that of the related protein RIG-1. When stimulated by dsRNA, MDA5 recruits the adaptor protein VISA and ultimately causes the activation of IRF-3 and NF- B (4). MDA5 and RIG-1 recognize different types of dsRNA, with MDA5 recognizing poly (I:C). MDA5-null mice were highly susceptible to infection with picornaviruses, which possess such sequences, demonstrating the importance of MDA5 in innate immunity (5). | | | Catalog # | M2772-76 | | Applications | Suitable for use in Western Blot. Other applications not tested. | | Recommended Dilution | Western Blot: 1-2ug/ml. See M2772-76A for control peptide. | | Optimal dilutions to be determined by the researcher. | | Positive Control | Daudi cell lysate | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Rabbit | | Source | Human | | Concentration | As reported | | Form | Supplied as a liquid in PBS, 0.02% sodium azide. | | Purity | Purified by immunoaffinity chromatography. | | Immunogen | 16 amino acid peptide from near the carboxy terminus of human MDA5 (GenBank accession no. NP_071451) | | Specificity | Recognizes human MDA5. Species Crossreactivity: Mouse. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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