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You are here:Home » Antibodies » Abs to DEAD Box Proteins » Anti -MDA5, CT (Interferon-induced Helicase C Domain-containing Protein 1, Clinically Amyopathic Dermatomyositis Autoantigen 140kD, CADM-140 Autoantigen Helicase with 2 CARD Domains, Helicard, Interferon-induced with Helicase C Domain Protein 1, Melanoma Differ

Anti -MDA5, CT (Interferon-induced Helicase C Domain-containing Protein 1, Clinically
Amyopathic Dermatomyositis Autoantigen 140kD, CADM-140 Autoantigen Helicase with 2 CARD
Domains, Helicard, Interferon-induced with Helicase C Domain Protein 1, Melanoma Differ

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Purified E B IH
The innate immune system detects viral infection by recognizing various viral components and triggers antiviral responses. Like the toll-like receptor 3 (TLR3), the melanoma differentiation-associated protein 5 (MDA5) recognizes double-stranded (ds) RNA, a molecular pattern associated with viral infection. MDA5, a member of the DEAD/DEAH-box RNA helicase family, consists of an amino-terminal caspase recruitment domain (CARD) and a carboxyl-terminal RNA helicase domain similar to that of the related protein RIG-1. When stimulated by dsRNA, MDA5 recruits the adaptor protein VISA and ultimately causes the activation of IRF-3 and NF-kB. MDA5 and RIG-1 recognize different types of dsRNA, with MDA5 recognizing poly (I:C). MDA5-null mice were highly susceptible to infection with picornaviruses, which possess such sequences, demonstrating the importance of MDA5 in innate immunity.
Catalog #M2772-76H
ApplicationsSuitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1-2ug/ml
Optimal dilutions to be determined by the researcher.
Positive ControlDaudi cell lysate
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~0.1mg/ml
FormSupplied as a liquid in PBS, 0.02% sodium azide.
PurityPurified
ImmunogenSynthetic peptide corresponding to 16aa from near the C-terminus of human MDA5.
SpecificityRecognizes human MDA5. Species Crossreactivity: mouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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